验证数据展示
经过测试的应用
| Positive WB detected in | HeLa cells, A549 cells |
| Positive IHC detected in | human breast cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Planning an IHC experiment? We recommend our IHCeasy ECE1 Ready-To-Use IHC Kit. ECE1 primary antibody included.
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
26088-1-AP targets ECE1 in WB, IHC, IF, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, IHC, ELISA Application Description |
| 文献引用应用 | WB, IF |
| 经测试反应性 | human |
| 文献引用反应性 | human, rat |
| 免疫原 |
CatNo: Ag23144 Product name: Recombinant human ECE1 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 335-425 aa of BC126257 Sequence: TAAELQTLAPAINWLPFLNTIFYPVEINESEPIVVYDKEYLEQISTLINTTDRCLLNNYMIWNLVRKTSSFLDQRFQDADEKFMEVMYGTK 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | endothelin converting enzyme 1 |
| 别名 | ECE, ECE 1, ECE1, endothelin converting enzyme 1 |
| 观测分子量 | 125-135 kDa |
| GenBank蛋白编号 | BC126257 |
| 基因名称 | ECE1 |
| Gene ID (NCBI) | 1889 |
| RRID | AB_2880371 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P42892 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
| Product Specific Protocols | |
|---|---|
| IHC protocol for ECE1 antibody 26088-1-AP | Download protocol |
| WB protocol for ECE1 antibody 26088-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Laryngoscope Investig Otolaryngol Hypoxia-inducible factor-1α contributes to the proliferation of cholesteatoma keratinocytes through regulating endothelin converting enzyme 1 expression
| ||
Adv Sci (Weinh) Astrocytic ET-1 System Determines Microglia Phenotype Following Spinal Cord Injury |
