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验证数据展示

  • WB analysis using 67179-1-Ig

    WB analysis using 67179-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67179-1-Ig (ECD antibody) at dilution of 1:100000 incubated at room temperature for 1.5 hours.

  • IHC staining of human renal cell carcinoma using 67179-1-Ig

    IHC staining of human renal cell carcinoma using 67179-1-Ig

    Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 67179-1-Ig (ECD antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with ECD antibody included
  • IHC staining of human renal cell carcinoma using 67179-1-Ig

    IHC staining of human renal cell carcinoma using 67179-1-Ig

    Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 67179-1-Ig (ECD antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with ECD antibody included

ECD Monoclonal antibody

ECD Monoclonal Antibody for WB, IHC, ELISA
Cat No. 67179-1-Ig

产品说明书

CloneNo. 1D5E8

宿主/亚型

Mouse / IgG1

种属反应性

Human, mouse, rat

应用

WB, IHC, ELISA

别名

ECD, GCR2, HSGT1, Protein ecdysoneless homolog, Protein SGT1, SGT1, Suppressor of GCR2

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHeLa cells, NIH/3T3 cells, HSC-T6 cells, Jurkat cells, JAR cells, NCCIT cells, HEK-293 cells
Positive IHC detected inhuman renal cell carcinoma tissue, human stomach cancer tissue, mouse bladder tissue, rat colon tissue, rat bladder tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy ECD Ready-To-Use IHC Kit. ECD primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:20000-1:100000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

67179-1-Ig targets ECD in WB, IHC, ELISA applications and shows reactivity with Human, mouse, rat samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 Human, mouse, rat
免疫原 ECD fusion protein Ag28674 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 ecdysoneless homolog (Drosophila)
别名 ECD, GCR2, HSGT1, Protein ecdysoneless homolog, Protein SGT1, SGT1, Suppressor of GCR2
计算分子量 73 kDa
观测分子量 70-73 kDa
GenBank蛋白编号BC000721
基因名称 ECD
Gene ID (NCBI) 11319
RRIDAB_2882475
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein G purification
UNIPROT IDO95905
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for ECD antibody 67179-1-IgDownload protocol
IHC protocol for ECD antibody 67179-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols