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验证数据展示

  • WB analysis using 29771-1-AP (same clone as 29771-1-PBS)

    WB analysis using 29771-1-AP (same clone as 29771-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 29771-1-AP (EBP50/NHERF-1 antibody) at dilution of 1:10000 and incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 29771-1-PBS in a different storage buffer formulation.

  • IHC staining of human placenta using 29771-1-AP (same clone as 29771-1-PBS)

    IHC staining of human placenta using 29771-1-AP (same clone as 29771-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 29771-1-AP (EBP50/NHERF-1 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 29771-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with SLC9A3R1 antibody included
  • IHC staining of human placenta using 29771-1-AP (same clone as 29771-1-PBS)

    IHC staining of human placenta using 29771-1-AP (same clone as 29771-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 29771-1-AP (EBP50/NHERF-1 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 29771-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with SLC9A3R1 antibody included
  • IHC staining of rat kidney using 29771-1-AP (same clone as 29771-1-PBS)

    IHC staining of rat kidney using 29771-1-AP (same clone as 29771-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 29771-1-AP (EBP50/NHERF-1 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 29771-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with SLC9A3R1 antibody included
  • IHC staining of rat kidney using 29771-1-AP (same clone as 29771-1-PBS)

    IHC staining of rat kidney using 29771-1-AP (same clone as 29771-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 29771-1-AP (EBP50/NHERF-1 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 29771-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with SLC9A3R1 antibody included
  • IF Staining of HeLa using 29771-1-AP (same clone as 29771-1-PBS)

    IF Staining of HeLa using 29771-1-AP (same clone as 29771-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using EBP50/NHERF-1 antibody (29771-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red). This data was developed using the same antibody clone with 29771-1-PBS in a different storage buffer formulation.

  • IF Staining of A549 using 29771-1-AP (same clone as 29771-1-PBS)

    IF Staining of A549 using 29771-1-AP (same clone as 29771-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed A549 cells using EBP50/NHERF-1 antibody (29771-1-AP) at dilution of 1:600 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). This data was developed using the same antibody clone with 29771-1-PBS in a different storage buffer formulation.

  • FC experiment of HepG2 using 29771-1-AP (same clone as 29771-1-PBS)

    FC experiment of HepG2 using 29771-1-AP (same clone as 29771-1-PBS)

    1X10^6 HepG2 cells were intracellularly stained with 0.4 ug Anti-HUMAN EBP50/NHERF-1 (29771-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer. This data was developed using the same antibody clone with 29771-1-PBS in a different storage buffer formulation.

EBP50/NHERF-1 Polyclonal antibody, PBS Only

EBP50/NHERF-1 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), Indirect ELISA
Cat No. 29771-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

别名

SLC9A3R1, NHERF-1, EBP50

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy SLC9A3R1 Ready-To-Use IHC Kit. SLC9A3R1 primary antibody included.

产品信息

29771-1-PBS targets EBP50/NHERF-1 in WB, IHC, IF/ICC, FC (Intra), Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, FC (Intra), Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag30878

Product name: Recombinant human SLC9A3R1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 260-358 aa of NM_004252

Sequence: TNGEIQKENSREALAEAALESPRPALVRSASSDTSEELNSQDSPPKQDSTAPSSTSSSDPILDFNISLAMAKERAHQKRSSKRAPQMDWSKKNELFSNL

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 solute carrier family 9 (sodium/hydrogen exchanger), member 3 regulator 1
别名 SLC9A3R1, NHERF-1, EBP50
计算分子量 39 kDa
观测分子量 50 kDa
GenBank蛋白编号NM_004252
基因名称 SLC9A3R1/EBP50/NHERF-1
Gene ID (NCBI) 9368
RRIDAB_2935478
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDO14745
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Na+/H+ exchanger regulatory factor 1 (NHERF-1, also known as EBP50 ezrin-binding protein of 50 kDa, SLCA9A3R1) is a phosphoprotein that assembles multiprotein complexes via two PDZ domains and a C-terminal ezrin-binding domain. NHERF-1 was initially recognized as a scaffolding protein that recruits membrane transporters/receptors and cytoplasmic signaling proteins into functional complexes localized at or near the plasma membrane in epithelial cells (PMID: 19308292).

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