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验证数据展示

  • IHC staining of human cervical cancer using 12968-1-AP

    IHC staining of human cervical cancer using 12968-1-AP

    Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 12968-1-AP (EBP antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human cervical cancer using 12968-1-AP

    IHC staining of human cervical cancer using 12968-1-AP

    Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 12968-1-AP (EBP antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • WB analysis using 12968-1-AP

    WB analysis using 12968-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 12968-1-AP (EBP antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

EBP Polyclonal antibody

EBP Polyclonal Antibody for WB, IHC, ELISA
Cat No. 12968-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, ELISA

别名

CPX, Cholestenol Delta-isomerase, Cholestenol Delta isomerase, CHO2, CDPX2

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inA549 cells, HeLa cells, Jurkat cells, mouse liver tissue
Positive IHC detected inhuman cervical cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:100-1:400
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

12968-1-AP targets EBP in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 EBP fusion protein Ag3630 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 emopamil binding protein (sterol isomerase)
别名 CPX, Cholestenol Delta-isomerase, Cholestenol Delta isomerase, CHO2, CDPX2
计算分子量 26 kDa
观测分子量 21 kDa
GenBank蛋白编号BC001572
基因名称 EBP
Gene ID (NCBI) 10682
RRIDAB_3085403
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ15125
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for EBP antibody 12968-1-APDownload protocol
IHC protocol for EBP antibody 12968-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols