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验证数据展示

  • WB analysis using 12371-1-AP (same clone as 12371-1-PBS)

    WB analysis using 12371-1-AP (same clone as 12371-1-PBS)

    human placenta tissue was subjected to SDS PAGE followed by western blot with 12371-1-AP (EBI3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12371-1-PBS in a different storage buffer formulation.

  • IP experiment of human placenta using 12371-1-AP (same clone as 12371-1-PBS)

    IP experiment of human placenta using 12371-1-AP (same clone as 12371-1-PBS)

    IP result of anti-EBI3 (IP:12371-1-AP, 4ug; Detection:12371-1-AP 1:800) with human placenta tissue lysate 600 ug. This data was developed using the same antibody clone with 12371-1-PBS in a different storage buffer formulation.

  • IHC staining of human spleen using 12371-1-AP (same clone as 12371-1-PBS)

    IHC staining of human spleen using 12371-1-AP (same clone as 12371-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human spleen tissue slide using 12371-1-AP (EBI3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12371-1-PBS in a different storage buffer formulation.

  • IHC staining of human spleen using 12371-1-AP (same clone as 12371-1-PBS)

    IHC staining of human spleen using 12371-1-AP (same clone as 12371-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human spleen tissue slide using 12371-1-AP (EBI3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12371-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

EBI3/IL-27B Polyclonal antibody, PBS Only

EBI3/IL-27B Polyclonal Antibody for WB, IHC, IP, Indirect ELISA
Cat No. 12371-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, pig

应用

WB, IHC, IP, Indirect ELISA

别名

EBI3, EBV induced gene 3 protein, EBV-induced gene 3 protein, Epstein Barr virus induced 3, Epstein-Barr virus-induced gene 3 protein

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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产品信息

12371-1-PBS targets EBI3/IL-27B in WB, IHC, IP, Indirect ELISA applications and shows reactivity with human, pig samples.

经测试应用 WB, IHC, IP, Indirect ELISA Application Description
经测试反应性 human, pig
免疫原

CatNo: Ag3051

Product name: Recombinant human EBI3 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-229 aa of BC015364

Sequence: MTPQLLLALVLWASCPPCSGRKGPPAALTLPRVQCRASRYPIAVDCSWTLPPAPNSTSPVSFIATYRLGMAARGHSWPCLQQTPTSTSCTITDVQLFSMAPYVLNVTAVHPWGSSSSFVPFITEHIIKPDPPEGVRLSPLAERQLQVQWEPPGSWPFPEIFSLKYWIRYKRQGAARFHRVGPIEATSFILRAVRPRARYYVQVAAQDLTDYGELSDWSLPATATMSLGK

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 Epstein-Barr virus induced 3
别名 EBI3, EBV induced gene 3 protein, EBV-induced gene 3 protein, Epstein Barr virus induced 3, Epstein-Barr virus-induced gene 3 protein
计算分子量 229 aa, 25 kDa
观测分子量 25-35 kDa
GenBank蛋白编号BC015364
基因名称 EBI3
Gene ID (NCBI) 10148
RRIDAB_10646470
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ14213
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

EBI3, a 34-kDa secreted glycoprotein, is a subunit in 2 distinct heterodimeric cytokines: IL-27 and IL-35. IL-27, composed of p28 (IL-27) and EBI3, can trigger signaling in T cells, B cells, and myeloid cells. IL-35, composed of EBI3 and the ubiquitously expressed p35 subunit of IL-12, is an inhibitory cytokine involved in regulatory T-cell function. EBI3 plays a role in regulating cell-mediated immune responses. (PMID: 8551575; 18033300; 17874423; 21931777)

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