验证数据展示
经过测试的应用
| Positive WB detected in | U2OS cells, Raji cells, 3T3-L1 cells |
| Positive IHC detected in | human stomach cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:200-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 2 publications below |
| IHC | See 1 publications below |
产品信息
14159-1-AP targets EBF1 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.
| 经测试应用 | WB, IHC, ELISA Application Description |
| 文献引用应用 | WB, IHC |
| 经测试反应性 | human, mouse |
| 文献引用反应性 | mouse |
| 免疫原 |
CatNo: Ag5341 Product name: Recombinant human EBF1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 4-350 aa of BC038805 Sequence: IQESIQRSGSSMKEEPLGSGMNAVRTWMQGAGVLDANTAAQSGVGLARAHFEKQPPSNLRKSNFFHFVLALYDRQGQPVEIERTAFVGFVEKEKEANSEKTNNGIHYRLQLLYSNGIRTEQDFYVRLIDSMTKQAIVYEGQDKNPEMCRVLLTHEIMCSRCCDKKSCGNRNETPSDPVIIDRFFLKFFLKCNQNCLKNAGNPRDMRRFQVVVSTTVNVDGHVLAVSDNMFVHNNSKHGRRARRLDPSEGTPSYLEHATPCIKAISPSEGWTTGGATVIIIGDNFFDGLQVIFGTMLVWSELITPHAIRVQTPPRHIPGVVEVTLSYKSKQFCKGTPGRFIYTALNEP 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | early B-cell factor 1 |
| 别名 | COE1, Early B cell factor, early B cell factor 1, EBF, EBF1, O/E 1, OE 1, OLF1, Transcription factor COE1 |
| 计算分子量 | 591 aa, 64 kDa |
| 观测分子量 | 57-67 kDa |
| GenBank蛋白编号 | BC038805 |
| 基因名称 | EBF1 |
| Gene ID (NCBI) | 1879 |
| RRID | AB_2231057 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q9UH73 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
EBF1, also named as O/E-1, OE-1, OLF1, COE1 and EBF, belongs to the COE family. It is a transcriptional activator which recognizes variations of the palindromic sequence 5'-ATTCCCNNGGGAATT-3'. EBF1 is a key factor for the specification of B-cell lineage.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for EBF1 antibody 14159-1-AP | Download protocol |
| IHC protocol for EBF1 antibody 14159-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
iScience Whitening of brown adipose tissue inhibits osteogenic differentiation via secretion of S100A8/A9 | ||
ACS Nano Arginine Supplementation Targeting Tumor-Killing Immune Cells Reconstructs the Tumor Microenvironment and Enhances the Antitumor Immune Response |
