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  • Featured Product
  • KD/KO Validated

RCAS1 Polyclonal antibody

RCAS1 Polyclonal Antibody for IF/ICC, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF/ICC, IHC, IP, WB, ELISA

Conjugate

Unconjugated

Cat No : 12255-1-AP

Print datasheet

Synonyms

EB9, EBAG9, PDAF, RCAS1

验证数据展示

  • WB analysis using 12255-1-AP

    WB analysis using 12255-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 12255-1-AP (RCAS1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IHC staining of human colon using 12255-1-AP

    IHC staining of human colon using 12255-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 12255-1-AP (RCAS1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with RCAS1 antibody included
  • WB analysis of HEK-293 using 12255-1-AP

    WB analysis of HEK-293 using 12255-1-AP

    WB result of RCAS1 antibody (12255-1-AP, 1:10000) with si-control and si-RCAS1 transfected HEK293 cells.

  • IP experiment of HeLa using 12255-1-AP

    IP experiment of HeLa using 12255-1-AP

    IP result of anti-RCAS1 (IP:12255-1-AP, 4ug; Detection:12255-1-AP 1:2000) with HeLa cells lysate 1720 ug.

  • IF Staining of HEK-293 using 12255-1-AP

    IF Staining of HEK-293 using 12255-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed HEK-293 cells using RCAS1 antibody (12255-1-AP) at dilution of 1:1200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

经过测试的应用

Positive WB detected inHEK-293 cells, HeLa cells, mouse heart tissue, rat heart tissue
Positive IP detected inHeLa cells
Positive IHC detected inhuman colon tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHEK-293 cells
Planning an IHC experiment? We recommend our IHCeasy RCAS1 Ready-To-Use IHC Kit. RCAS1 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:250-1:1000
Immunofluorescence (IF)IF : 1:600-1:2400
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

12255-1-AP targets RCAS1 in WB, IP, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications IF/ICC, IHC, IP, WB, ELISA
Tested Reactivity human, mouse, rat
Immunogen RCAS1 fusion protein Ag2905 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name estrogen receptor binding site associated, antigen, 9
Synonyms EB9, EBAG9, PDAF, RCAS1
Calculated Molecular Weight 213 aa, 24 kDa
Observed Molecular Weight 32 kDa
GenBank Accession NumberBC017729
Gene Symbol EBAG9
Gene ID (NCBI) 9166
RRIDAB_2246420
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO00559
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Estrogen receptor-binding fragment-associated antigen 9 (EBAG9) gene was identified as an estrogen-responsive gene. The gene product, receptor-binding cancer antigen expressed on SiSo cells (RCAS1), is associated with aggressive characteristics and poor overall survival for 15 different human malignancies. The correlation between RCAS1 expression and several clinicopathological variables, including tumor size, clinical stage, invasion depth and lymph node metastasis highlights this molecule's clinical significance. Expression of RCAS1 in tumor cells plays an important role in evasion from host immune system resulting tumor progression, invasion and metastasis. Further exploration of RCAS1 biological function will facilitate development of novel therapeutic strategies that target RCAS1.

实验方案

Product Specific Protocols
WB protocol for RCAS1 antibody 12255-1-APDownload protocol
IHC protocol for RCAS1 antibody 12255-1-APDownload protocol
IF protocol for RCAS1 antibody 12255-1-APDownload protocol
IP protocol for RCAS1 antibody 12255-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols