验证数据展示
经过测试的应用
| Positive WB detected in | mouse liver mitochondria tissue |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
17539-1-AP targets EARS2 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | human, mouse, rat |
| 免疫原 |
CatNo: Ag11594 Product name: Recombinant human EARS2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 184-534 aa of BC040013 Sequence: KPAIRFRLEQVVPAFQDLVYGWNRHEVASVEGDPVIMKSDGFPTYHLACVVDDHHMGISHVLRGSEWLVSTAKHLLLYQALGWQPPHFAHLPLLLNRDGSKLSKRQGDVFLEHFAADGFLPDSLLDIITNCGSGFAENQMGRTLPELITQFNLTQVTCHSALLDLEKLPEFNRLHLQRLVSNESQRRQLVGKLQVLVEEAFGCQLQNRDVLNPVYVERILLLRQGHICRLQDLVSPVYSYLWTRPAVGRAQLDAISEKVDVIAKRVLGLLERSGMSLTQDMLNGELKKLSEGLEGTKYSNVMKLLRMALSGQQVRQGHGLDCSLEPLIDPLNLHFLAGTELNIEYTKVNET 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | glutamyl-tRNA synthetase 2, mitochondrial (putative) |
| 别名 | EARS2, GluRS, Glutamate tRNA ligase, KIAA1970, MSE1 |
| 计算分子量 | 534 aa, 60 kDa |
| 观测分子量 | 59 kDa |
| GenBank蛋白编号 | BC040013 |
| 基因名称 | EARS2 |
| Gene ID (NCBI) | 124454 |
| RRID | AB_2878414 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q5JPH6 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
EARS2 encodes mitochondrial glutamyltRNA synthetase 2. Two isoforms were produced by alternative splicing with predicted MW of 58 and 60 kDa according to UniProt, and this antibody recognises both.
