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验证数据展示

  • WB analysis of HEK-293 using 16931-1-AP

    WB analysis of HEK-293 using 16931-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 16931-1-AP (EAPP Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • IF Staining of HeLa using 16931-1-AP

    IF Staining of HeLa using 16931-1-AP

    Immunofluorescent analysis of (10% Formaldehyde) fixed HeLa cells using 16931-1-AP (EAPP antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

EAPP Polyclonal antibody

EAPP Polyclonal Antibody for WB, IF/ICC, ELISA
Cat No. 16931-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IF/ICC, ELISA

别名

E2F-associated phosphoprotein, E2F associated phosphoprotein, C14orf11, BM-036, BM036

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inHEK-293 cells, HeLa cells
Positive IF/ICC detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy EAPP Ready-To-Use IHC Kit. EAPP primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

16931-1-AP targets EAPP in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag10403

Product name: Recombinant human EAPP protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-285 aa of BC001245

Sequence: MNRLPDDYDPYAVEEPSDEEPALSSSEDEVDVLLHGTPDQKRKLIRECLTGESESSSEDEFEKEMEAELNSTMKTMEDKLSSLGTGSSSGNGKVATAPTRYYDDIYFDSDSEDEDRAVQVTKKKKKKQHKIPTNDELLYDPEKDNRDQAWVDAQRRGYHGLGPQRSREQQPVPNSDAVLNCPACMTTLCLDCQRHESYKTQYRAMFVMNCSINKEEVLRYKASENRKKRRVHKKMRSNQEDAAEKAETDVEEIYHPVMCTECSTEVAVYDKDEVFHFFNVLASHS

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 E2F-associated phosphoprotein
别名 E2F-associated phosphoprotein, E2F associated phosphoprotein, C14orf11, BM-036, BM036
计算分子量 285 aa, 33 kDa
观测分子量 40-45 kDa
GenBank蛋白编号BC001245
基因名称 EAPP
Gene ID (NCBI) 55837
RRIDAB_2878334
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ56P03
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for EAPP antibody 16931-1-APDownload protocol
IF protocol for EAPP antibody 16931-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols