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验证数据展示

  • WB analysis of HEK-293 using 11172-1-AP (same clone as 11172-1-PBS)

    WB analysis of HEK-293 using 11172-1-AP (same clone as 11172-1-PBS)

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 11172-1-AP (EAF2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11172-1-PBS in a different storage buffer formulation.

  • IHC staining of human breast cancer using 11172-1-AP (same clone as 11172-1-PBS)

    IHC staining of human breast cancer using 11172-1-AP (same clone as 11172-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 11172-1-AP (EAF2 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11172-1-PBS in a different storage buffer formulation.

  • IHC staining of human breast cancer using 11172-1-AP (same clone as 11172-1-PBS)

    IHC staining of human breast cancer using 11172-1-AP (same clone as 11172-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 11172-1-AP (EAF2 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11172-1-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 11172-1-AP (same clone as 11172-1-PBS)

    IF Staining of HepG2 using 11172-1-AP (same clone as 11172-1-PBS)

    Immunofluorescent analysis of HepG2 cells using 11172-1-AP (EAF2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 11172-1-PBS in a different storage buffer formulation.

EAF2 Polyclonal antibody, PBS Only

EAF2 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 11172-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, Indirect ELISA

别名

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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产品信息

11172-1-PBS targets EAF2 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag1637

Product name: Recombinant human EAF2 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-260 aa of BC014209

Sequence: MNSAAGFSHLDRRERVLKLGESFEKQPRCAFHTVRYDFKPASIDTSSEGYLEVGEGEQVTITLPNIEGSTPPVTVFKGSKKPYLKECILIINHDTGECRLEKLSSNITVKKTRVEGSSKIQYRKEQQQQQMWNSARTPNLVKHSPSEDKMSPASPIDDIERELKAEASLMDQMSSCDSSSDSKSSSSSSSEDSSSDSEDEDCKSSTSDTGNCVSGHPTMTQYRIPDIDASHNRFRDNSGLLMNTLRNDLQLSESGSDSDD

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 ELL associated factor 2
别名
计算分子量 42 kDa
观测分子量 45 kDa
GenBank蛋白编号BC014209
基因名称 EAF2
Gene ID (NCBI) 55840
RRIDAB_2097560
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ96CJ1
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

ELL associated factor 1 and ELL associated factor 2 (EAF1/2 factors) are reported to play important roles in tumor suppression and embryogenesis. The tumor suppressor EAF2 is regulated by androgen signaling and associated with prostate cancer. Eaf factors have been revealed to modulate mesoderm and neural patterning by inhibiting canonical Wnt signaling, and high activity of canonical Wnt/β-catenin is revealed in eafs morphants (PMID: 23364330). Catalog#11172-1-AP is a rabbit polyclonal antibody raised against the full-length of human EAF2.

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