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验证数据展示

  • WB analysis of A375 using 12876-1-AP (same clone as 12876-1-PBS)

    WB analysis of A375 using 12876-1-AP (same clone as 12876-1-PBS)

    A375 cells were subjected to SDS PAGE followed by western blot with 12876-1-AP (EAAT4 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12876-1-PBS in a different storage buffer formulation.

  • IF Staining of mouse cerebellum using 12876-1-AP (same clone as 12876-1-PBS)

    IF Staining of mouse cerebellum using 12876-1-AP (same clone as 12876-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed frozen OCT-embedded mouse cerebellum tissue using EAAT4 antibody (12876-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 12876-1-PBS in a different storage buffer formulation.

  • IF Staining of mouse cerebellum using 12876-1-AP (same clone as 12876-1-PBS)

    IF Staining of mouse cerebellum using 12876-1-AP (same clone as 12876-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed frozen OCT-embedded mouse cerebellum tissue using EAAT4 antibody (12876-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 12876-1-PBS in a different storage buffer formulation.

EAAT4 Polyclonal antibody, PBS Only

EAAT4 Polyclonal Antibody for WB, IF-Fro, Indirect ELISA
Cat No. 12876-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IF-Fro, Indirect ELISA

别名

SLC1A6, Sodium-dependent glutamate/aspartate transporter

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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产品信息

12876-1-PBS targets EAAT4 in WB, IF-Fro, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IF-Fro, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag3554

Product name: Recombinant human SLC1A6 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 149-271 aa of BC028721

Sequence: MVTIIHPGKGSKEGLHREGRIETIPTADAFMDLIRNMFPPNLVEACFKQFKTQYSTRVVTRTMVRTENGSEPGASMPPPFSVENGTSFLENVTRALGTLQEMLSFEETVPVPGSANGINALGL

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 solute carrier family 1 (high affinity aspartate/glutamate transporter), member 6
别名 SLC1A6, Sodium-dependent glutamate/aspartate transporter
计算分子量 61.6 kDa
观测分子量 60-70 kDa
GenBank蛋白编号BC028721
基因名称 EAAT4
Gene ID (NCBI) 6511
RRIDAB_10642147
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDP48664
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Excitatory amino acid transporter 4 (EAAT4) is a neuronal glutamate transporter responsible for the reuptake of synaptically released glutamate. The EAAT4 protein is highly enriched in Purkinje cells of the cerebellum, although it is not restricted to these cells (18770868). While the predicted molecular weight of EAAT4 is 62 kDa, considerable heterogeneity in transporter size has been reported in the brain with molecular weights ranging from 50 to 120 kDa as a result of differential glycosylation and/or phosphorylation (24056007). This antibody recognizes 60-70 kDa of EAAT4 in mouse brain and several other lysate.

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