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验证数据展示

  • WB analysis of mouse ovary using 12686-1-AP (same clone as 12686-1-PBS)

    WB analysis of mouse ovary using 12686-1-AP (same clone as 12686-1-PBS)

    mouse ovary tissue were subjected to SDS PAGE followed by western blot with 12686-1-AP (EAAT3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12686-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse brain using 12686-1-AP (same clone as 12686-1-PBS)

    IHC staining of mouse brain using 12686-1-AP (same clone as 12686-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 12686-1-AP (EAAT3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12686-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with SLC1A1/EAAT3 antibody included
  • IHC staining of mouse brain using 12686-1-AP (same clone as 12686-1-PBS)

    IHC staining of mouse brain using 12686-1-AP (same clone as 12686-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 12686-1-AP (EAAT3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12686-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with SLC1A1/EAAT3 antibody included
  • Featured Product
  • KD/KO Validated

EAAT3 Polyclonal antibody, PBS Only

EAAT3 Polyclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 12686-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, Indirect ELISA

别名

SLC1A1, EAAT 3, Excitatory amino-acid carrier 1, HEAAC1, Slc1 a1

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy SLC1A1/EAAT3 Ready-To-Use IHC Kit. SLC1A1/EAAT3 primary antibody included.

产品信息

12686-1-PBS targets EAAT3 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag3379

Product name: Recombinant human SLC1A1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 430-524 aa of BC033040

Sequence: AEDVTLIIAVDWLLDRFRTMVNVLGDAFGTGIVEKLSKKELEQMDVSSEVNIVNPFALESTILDNEDSDTKKSYVNGGFAVDKSDTISFTQTSQF

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 solute carrier family 1 (neuronal/epithelial high affinity glutamate transporter, system Xag), member 1
别名 SLC1A1, EAAT 3, Excitatory amino-acid carrier 1, HEAAC1, Slc1 a1
计算分子量 524 aa, 57 kDa
观测分子量 66 kDa
GenBank蛋白编号BC033040
基因名称 EAAT3
Gene ID (NCBI) 6505
RRIDAB_2188001
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDP43005
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

EAAT3 (Excitatory Amino Acid Transporter 3), encoded by human SLC1A1 (Gene ID: 6505), is a neuronal high-affinity glutamate transporter of the SLC1 family. It is expressed mainly in CNS neurons, and also in intestinal and renal epithelial cells. EAAT3 is the only EAAT subtype that efficiently transports L-cysteine, supplying substrate for neuronal glutathione (GSH) synthesis and antioxidant defense. Its transport cycle couples uptake of L-glutamate, L-aspartate, or L-cysteine to 3 Na⁺/1 H⁺ co-transport and 1 K⁺ counter-transport. EAAT3 also contributes to metabolic reprogramming in activated B cells and cancer cells, and can transport the oncometabolite R-2-hydroxyglutarate (R-2HG). SLC1A1 mutations are linked to dicarboxylic aminoaciduria and schizophrenia susceptibility.

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