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验证数据展示

  • WB analysis using 67083-1-Ig (same clone as 67083-1-PBS)

    WB analysis using 67083-1-Ig (same clone as 67083-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67083-1-Ig (SLC1A2 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67083-1-PBS in a different storage buffer formulation.

  • Sample test of MP51374-1

    Sample test of MP51374-1

    Sample test of MP51374-1, SLC1A2 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 67083-2-PBS. Detection antibody: 67083-1-PBS.

  • Cytometric bead array standard curve of MP51374-1

    Cytometric bead array standard curve of MP51374-1

    Cytometric bead array standard curve of MP51374-1, SLC1A2 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 67083-2-PBS. Detection antibody: 67083-1-PBS. Standard:Ag19816. Range: 0.098-100 ng/mL.

EAAT2 Monoclonal antibody, PBS Only (Detector)

EAAT2 Monoclonal Antibody for WB, Cytometric bead array, Indirect ELISA, Sample test
Cat No. 67083-1-PBS

产品说明书

CloneNo. 1C8D10

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse, rat

应用

WB, Cytometric bead array, Indirect ELISA, Sample test

别名

GLT1, SLC1A2, 1C8D10, GLT 1, Sodium-dependent glutamate/aspartate transporter 2

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy EAAT2/SLC1A2 Ready-To-Use IHC Kit. EAAT2/SLC1A2 primary antibody included.

产品信息

67083-1-PBS targets EAAT2 as part of a matched antibody pair:

MP51374-1: 67083-2-PBS capture and 67083-1-PBS detection (validated in Cytometric bead array)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

经测试应用 WB, Cytometric bead array, Indirect ELISA, Sample test Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag19816

Product name: Recombinant human SLC1A2 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 460-574 aa of BC132768

Sequence: PTEDISLLVAVDWLLDRMRTSVNVVGDSFGAGIVYHLSKSELDTIDSQHRVHEDIEMTKTQSIYDDMKNHRESNSNQCVYAAHNSVIVDECKVTLAANGKSADCSVEEEPWKREK

种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 solute carrier family 1 (glial high affinity glutamate transporter), member 2
别名 GLT1, SLC1A2, 1C8D10, GLT 1, Sodium-dependent glutamate/aspartate transporter 2
计算分子量 574 aa, 62 kDa
观测分子量 60-70 kDa
GenBank蛋白编号BC132768
基因名称 EAAT2
Gene ID (NCBI) 6506
RRIDAB_2882391
偶联类型 Unconjugated
形式Liquid
纯化方式Protein G purification
UNIPROT IDP43004
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

EAAT2 (also known as GLT-1), is encoded by SLC1A2 and is a glutamate transporter which can clear the majority of extracellular glutamate in brain and prevent brain seizures.Three isoforms of EAAT2 exist due to the alternative splicing. In addition to the 65-70 kDa monomer form, 130-150 kDa dimer of EAAT2 can also be observed on SDS-PAGE.(PMID: 22114258)

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