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验证数据展示

  • WB analysis using 67812-1-Ig (same clone as 67812-1-PBS)

    WB analysis using 67812-1-Ig (same clone as 67812-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67812-1-Ig (E2F4 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Beta Actin Monoclonal antibody (HRP-66009) as loading control. This data was developed using the same antibody clone with 67812-1-PBS in a different storage buffer formulation.

  • WB analysis using 67812-1-Ig (same clone as 67812-1-PBS)

    WB analysis using 67812-1-Ig (same clone as 67812-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67812-1-Ig (E2F4 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Beta Actin Monoclonal antibody (HRP-66009) as loading control. This data was developed using the same antibody clone with 67812-1-PBS in a different storage buffer formulation.

  • WB analysis using 67812-1-Ig (same clone as 67812-1-PBS)

    WB analysis using 67812-1-Ig (same clone as 67812-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67812-1-Ig (E2F4 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67812-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO验证

E2F4 Monoclonal antibody, PBS Only

E2F4 Monoclonal Antibody for WB, Indirect ELISA
Cat No. 67812-1-PBS

产品说明书

CloneNo. 2D1E1

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse, rat

应用

WB, Indirect ELISA

别名

Transcription factor E2F4, E2F-4, E2F 4, 2D1E1

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

67812-1-PBS targets E2F4 in WB, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 E2F4 fusion protein Ag28533 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 E2F transcription factor 4, p107/p130-binding
别名 Transcription factor E2F4, E2F-4, E2F 4, 2D1E1
计算分子量 44 kDa
GenBank蛋白编号BC033180
基因名称 E2F4
Gene ID (NCBI) 1874
RRIDAB_2918575
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein G purification
UNIPROT IDQ16254
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Transcription factor E2F4 (E2F4) is a transcription activator that binds DNA cooperatively with DP proteins through the E2 recognition site 5'-TTTC[CG]CGC-3' found in the promoter region of a number of genes whose products are involved in cell cycle regulation or in DNA replication. The DRTF1/E2F complex functions in the control of cell-cycle progression from G1 to S phase. E2F-4 binds with high affinity to RBL1 and RBL2. In some instances, can also bind RB protein.The observed molecular weight of 60-70 kDa is the phosphorylation of E2F4 protein.