• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis using 67812-1-Ig (same clone as 67812-1-PBS)

    WB analysis using 67812-1-Ig (same clone as 67812-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67812-1-Ig (E2F4 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Beta Actin Monoclonal antibody (HRP-66009) as loading control. This data was developed using the same antibody clone with 67812-1-PBS in a different storage buffer formulation.

  • WB analysis using 67812-1-Ig (same clone as 67812-1-PBS)

    WB analysis using 67812-1-Ig (same clone as 67812-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67812-1-Ig (E2F4 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Beta Actin Monoclonal antibody (HRP-66009) as loading control. This data was developed using the same antibody clone with 67812-1-PBS in a different storage buffer formulation.

  • WB analysis using 67812-1-Ig (same clone as 67812-1-PBS)

    WB analysis using 67812-1-Ig (same clone as 67812-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67812-1-Ig (E2F4 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67812-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO验证

E2F4 Monoclonal antibody, PBS Only

E2F4 Monoclonal Antibody for WB, Indirect ELISA
Cat No. 67812-1-PBS

产品说明书

CloneNo. 2D1E1

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse, rat

应用

WB, Indirect ELISA

别名

2D1E1, E2F 4, E2F-4, Transcription factor E2F4

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格价格库存

点击咨询售前客服

点击咨询售后客服

产品信息

67812-1-PBS targets E2F4 in WB, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag28533

Product name: Recombinant human E2F4 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-413 aa of BC033180

Sequence: MAEAGPQAPPPPGTPSRHEKSLGLLTTKFVSLLQEAKDGVLDLKLAADTLAVRQKRRIYDITNVLEGIGLIEKKSKNSIQWKGVGPGCNTREIADKLIELKAEIEELQQREQELDQHKVWVQQSIRNVTEDVQNSCLAYVTHEDICRCFAGDTLLAIRAPSGTSLEVPIPEGLNGQKKYQIHLKSVSGPIEVLLVNKEAWSSPPVAVPVPPPEDLLQSPSAVSTPPPLPKPALAQSQEASRPNSPQLTPTAVPGSAEVQGMAGPAAEITVSGGPGTDSKDSGELSSLPLGPTTLDTRPLQSSALLDSSSSSSSSSSSSSNSNSSSSSGPNPSTSFEPIKADPTGVLELPKELSEIFDPTRECMSSELLEELMSSEVFAPLLRLSPPPGDHDYIYNLDESEGVCDLFDVPVLNL

种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 E2F transcription factor 4, p107/p130-binding
别名 2D1E1, E2F 4, E2F-4, Transcription factor E2F4
计算分子量 44 kDa
观测分子量60-70 kDa
GenBank蛋白编号BC033180
基因名称 E2F4
Gene ID (NCBI) 1874
RRIDAB_2918575
偶联类型 Unconjugated
形式Liquid
纯化方式Protein G purification
UNIPROT IDQ16254
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Transcription factor E2F4 (E2F4) is a transcription activator that binds DNA cooperatively with DP proteins through the E2 recognition site 5'-TTTC[CG]CGC-3' found in the promoter region of a number of genes whose products are involved in cell cycle regulation or in DNA replication. The DRTF1/E2F complex functions in the control of cell-cycle progression from G1 to S phase. E2F-4 binds with high affinity to RBL1 and RBL2. In some instances, can also bind RB protein.The observed molecular weight of 60-70 kDa is the phosphorylation of E2F4 protein.