验证数据展示
经过测试的应用
| Positive WB detected in | SGC-7901 cells, SKOV-3 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
27615-1-AP targets E2F3 in WB, IHC, ELISA applications and shows reactivity with Human samples.
| 经测试应用 | WB, ELISA Application Description |
| 文献引用应用 | WB, IHC |
| 经测试反应性 | Human |
| 文献引用反应性 | human, mouse, rat |
| 免疫原 |
CatNo: Ag26367 Product name: Recombinant human E2F3 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 54-155 aa of NM_001243076 Sequence: MPGAYIQILTTNTSTTSCSSSLQSGAVAAGPLLPSAPGAEQTAGSLLYTTPHGPSSRAGLLQQPPALGRGGSGGGGGPPAKRRLELGESGHQYLSDGLKTPKG 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | E2F transcription factor 3 |
| 别名 | E2F 3, E2F transcription factor 3, E2F3, KIAA0075, Transcription factor E2F3 |
| 计算分子量 | 49 kDa |
| 观测分子量 | 60-65 kDa |
| GenBank蛋白编号 | NM_001243076 |
| 基因名称 | E2F3 |
| Gene ID (NCBI) | 1871 |
| RRID | AB_2880926 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | O00716 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for E2F3 antibody 27615-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Mol Ther Nucleic Acids miRNA in food simultaneously controls animal viral disease and human tumorigenesis. | ||
Mol Ther Nucleic Acids Shrimp miR-34 from Shrimp Stress Response to Virus Infection Suppresses Tumorigenesis of Breast Cancer. | ||
Oncotarget SNHG1 promotes cell proliferation by acting as a sponge of miR-145 in colorectal cancer. | ||
Sci China Life Sci rtcisE2F promotes the self-renewal and metastasis of liver tumor-initiating cells via N6-methyladenosine-dependent E2F3/E2F6 mRNA stability.
| ||
Phytomedicine Apigenin inhibits the growth of colorectal cancer through down-regulation of E2F1/3 by miRNA-215-5p. | ||
Int J Mol Med MicroRNA‑145‑5p inhibits osteosarcoma cell proliferation by targeting E2F transcription factor 3. |
