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验证数据展示

  • WB analysis using 66515-1-Ig (same clone as 66515-1-PBS)

    WB analysis using 66515-1-Ig (same clone as 66515-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66515-1-Ig (E2F1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66515-1-PBS in a different storage buffer formulation.

  • WB analysis of HepG2 using 66515-1-Ig (same clone as 66515-1-PBS)

    WB analysis of HepG2 using 66515-1-Ig (same clone as 66515-1-PBS)

    WB result of E2F1 antibody (66515-1-Ig; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-E2F1 transfected HepG2 cells. This data was developed using the same antibody clone with 66515-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO验证

E2F1 Monoclonal antibody, PBS Only

E2F1 Monoclonal Antibody for WB, Indirect ELISA
Cat No. 66515-1-PBS

产品说明书

CloneNo. 5D7G8

宿主/亚型

Mouse / IgG2b

种属反应性

human, rat

应用

WB, Indirect ELISA

别名

5D7G8, E2F 1, E2F-1, pRB-binding protein E2F-1, RBAP-1

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

66515-1-PBS targets E2F1 in WB, Indirect ELISA applications and shows reactivity with human, rat samples.

经测试应用 WB, Indirect ELISA Application Description
经测试反应性 human, rat
免疫原

CatNo: Ag17363

Product name: Recombinant human E2F1 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 88-438 aa of BC050369

Sequence: VKRRLDLETDHQYLAESSGPARGRGRHPGKGVKSPGEKSRYETSLNLTTKRFLELLSHSADGVVDLNWAAEVLKVQKRRIYDITNVLEGIQLIAKKSKNHIQWLGSHTTVGVGGRLEGLTQDLRQLQESEQQLDHLMNICTTQLRLLSEDTDSQRLAYVTCQDLRSIADPAEQMVMVIKAPPETQLQAVDSSENFQISLKSKQGPIDVFLCPEETVGGISPGKTPSQEVTSEEENRATDSATIVSPPPSSPPSSLTTDPSQSLLSLEQEPLLSRMGSLRAPVDEDRLSPLVAADSLLEHVREDFSGLLPEEFISLSPPHEALDYHFGLEEGEGIRDLFDCDFGDLTPLDF

种属同源性预测
宿主/亚型 Mouse / IgG2b
抗体类别 Monoclonal
产品类型 Antibody
全称 E2F transcription factor 1
别名 5D7G8, E2F 1, E2F-1, pRB-binding protein E2F-1, RBAP-1
计算分子量 437 aa, 47 kDa
观测分子量 55-60 kDa
GenBank蛋白编号BC050369
基因名称 E2F1
Gene ID (NCBI) 1869
RRIDAB_2881878
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDQ01094
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Transcription factor E2F1 (E2F1), also known as RBBP3, is a transcription activator that binds DNA cooperatively with dp proteins through the E2 recognition site, 5'-TTTC[CG]CGC-3' found in the promoter region of a number of genes whose products are involved in cell cycle regulation or in DNA replication. The DRTF1/E2F complex functions in the control of cell-cycle progression from G1 to S phase. E2F-1 binds preferentially RB1 protein, in a cell-cycle dependent manner. It can mediate both cell proliferation and p53-dependent apoptosis. The calculated molecular weight of E2F1 is 47 kDa, but the sumoylated E2F1 is bout 55-60 kDa.