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验证数据展示

  • WB analysis of PC-3 using 60335-1-Ig

    WB analysis of PC-3 using 60335-1-Ig

    PC-3, MKN-45, SGC-7901 cells were subjected to SDS PAGE followed by western blot with 60335-1-Ig (E-cadherin Antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • WB analysis of A431 using 60335-1-Ig

    WB analysis of A431 using 60335-1-Ig

    WB result of E-cadherin antibody (60335-1-Ig; 1:5000; incubated at room temperature for 1.5 hours) with sh-Control and sh-E-cadherin transfected A431 cells.

  • WB analysis using 60335-1-Ig

    WB analysis using 60335-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 60335-1-Ig (E-cadherin antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IF Staining of human kidney using 60335-1-Ig

    IF Staining of human kidney using 60335-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed human kidney tissue using E-cadherin antibody (60335-1-Ig, Clone: 6B11F11 ) at dilution of 1:300 and CoraLite®594-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), Podocalyxin antibody(18150-1-AP, green). DNA was stained by DAPI (blue).

  • IF Staining of human breast cancer using 60335-1-Ig

    IF Staining of human breast cancer using 60335-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed human breast cancer tissue using E-cadherin antibody (60335-1-Ig, Clone: 6B11F11 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

  • IF Staining of MCF-7 using 60335-1-Ig

    IF Staining of MCF-7 using 60335-1-Ig

    Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using E-cadherin antibody (60335-1-Ig, Clone: 6B11F11 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

  • IHC staining of human breast cancer using 60335-1-Ig

    IHC staining of human breast cancer using 60335-1-Ig

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 60335-1-Ig (E-cadherin antibody) at dilution of 1:2000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with E-cadherin antibody included
  • IHC staining of human breast cancer using 60335-1-Ig

    IHC staining of human breast cancer using 60335-1-Ig

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 60335-1-Ig (E-cadherin antibody) at dilution of 1:2000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with E-cadherin antibody included
  • IHC staining of human breast cancer using 60335-1-Ig

    IHC staining of human breast cancer using 60335-1-Ig

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 60335-1-Ig (E-cadherin antibody) at dilution of 1:8000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with E-cadherin antibody included
  • IHC staining of human breast cancer using 60335-1-Ig

    IHC staining of human breast cancer using 60335-1-Ig

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 60335-1-Ig (E-cadherin antibody) at dilution of 1:10000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with E-cadherin antibody included
  • IHC staining of human liver cancer using 60335-1-Ig

    IHC staining of human liver cancer using 60335-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 60335-1-Ig (E-cadherin antibody) at dilution of 1:10000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with E-cadherin antibody included
  • IHC staining of rat colon using 60335-1-Ig

    IHC staining of rat colon using 60335-1-Ig

    Immunohistochemical analysis of paraffin-embedded rat colon tissue slide using 60335-1-Ig (E-cadherin antibody) at dilution of 1:10000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with E-cadherin antibody included
  • IHC staining of human colon using 60335-1-Ig

    IHC staining of human colon using 60335-1-Ig

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 60335-1-Ig (E-cadherin antibody) at dilution of 1:1 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with E-cadherin antibody included
  • IF Staining of mouse kidney using 60335-1-Ig

    IF Staining of mouse kidney using 60335-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse kidney tissue using E-cadherin antibody (60335-1-Ig, Clone: 6B11F11 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1), Podocalyxin antibody (87582-2-RR, Clone: 252967A3, Magenta), CoraLite®594 ACE2 antibody (CL594-21115, red). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of mouse kidney using 60335-1-Ig

    IF Staining of mouse kidney using 60335-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse kidney tissue using E-cadherin antibody (60335-1-Ig, Clone: 6B11F11 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1), CoraLite®594 ACE2 antibody (CL594-21115, red), Podocalyxin antibody (87582-2-RR, Clone: 252967A3, Magenta). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of mouse kidney using 60335-1-Ig

    IF Staining of mouse kidney using 60335-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse kidney tissue using E-cadherin antibody (60335-1-Ig, Clone: 6B11F11 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1), CoraLite®594 ACE2 antibody (CL594-21115, red), Podocalyxin antibody (87582-2-RR, Clone: 252967A3, Magenta). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of mouse brain using 60335-1-Ig

    IF Staining of mouse brain using 60335-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed frozen OCT-embedded mouse brain tissue using E-cadherin antibody (60335-1-Ig, Clone: 6B11F11 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1).

  • IF Staining of mouse breast cancer using 60335-1-Ig

    IF Staining of mouse breast cancer using 60335-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed mouse breast cancer using E-cadherin antibody (60335-1-Ig, Clone: 6B11F11 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1).

  • Featured Product
  • KD/KO Validated

E-cadherin Monoclonal antibody

E-cadherin Monoclonal Antibody for WB, IHC, IF/ICC, IF-P, IF-Fro, ELISA
Cat No. 60335-1-Ig

产品说明书

CloneNo. 6B11F11
引用文献(288)

宿主/亚型

Mouse / IgG2b

种属反应性

human, mouse, rat, pig and More (2)

应用

WB, IHC, IF/ICC, IF-P, IF-Fro, ELISA and More (1)

别名

6B11F11, Arc 1, Cadherin 1, Cadherin-1, CAM 120/80

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inPC-3 cells, A431 cells, MCF-7 cells, pig brain tissue, MKN-45 cells, SGC-7901 cells
Positive IHC detected inhuman breast cancer tissue, human colon tissue, rat colon tissue, rat stomach tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inhuman breast cancer tissue, human kidney tissue, mouse kidney tissue
Positive IF-Fro detected inmouse brain tissue
Positive IF/ICC detected inMCF-7 cells, mouse breast cancer, HaCaT cells
Planning an IHC experiment? We recommend our IHCeasy E-cadherin Ready-To-Use IHC Kit. E-cadherin primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488, CoraLite®555 and CoraLite®594 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:8000
Immunohistochemistry (IHC)IHC : 1:1000-1:4000
Immunofluorescence (IF)-PIF-P : 1:200-1:800
Immunofluorescence (IF)-FROIF-FRO : 1:200-1:800
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

60335-1-Ig targets E-cadherin in WB, IHC, IF/ICC, IF-P, IF-Fro, ELISA applications and shows reactivity with human, mouse, rat, pig samples.

经测试应用 WB, IHC, IF/ICC, IF-P, IF-Fro, ELISA Application Description
文献引用应用WB, IHC, IF
经测试反应性 human, mouse, rat, pig
文献引用反应性human, mouse, rat, pig, canine, monkey
免疫原

CatNo: Ag15085

Product name: Recombinant human E-cadherin protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 373-622 aa of BC141838

Sequence: PIFNPTTYKGQVPENEANVVITTLKVTDADAPNTPAWEAVYTILNDDGGQFVVTTNPVNNDGILKTAKGLDFEAKQQYILHVAVTNVVPFEVSLTTSTATVTVDVLDVNEAPIFVPPEKRVEVSEDFGVGQEITSYTAQEPDTFMEQKITYRIWRDTANWLEINPDTGAISTRAELDREDFEHVKNSTYTALIIATDNGSPVATGTGTLLLILSDVNDNAPIPEPRTIFFCERNPKPQVINIIDADLPPI

种属同源性预测
宿主/亚型 Mouse / IgG2b
抗体类别 Monoclonal
产品类型 Antibody
全称 cadherin 1, type 1, E-cadherin (epithelial)
别名 6B11F11, Arc 1, Cadherin 1, Cadherin-1, CAM 120/80
计算分子量 882 aa, 97 kDa
观测分子量 120 kDa
GenBank蛋白编号BC141838
基因名称 E-cadherin
Gene ID (NCBI) 999
RRIDAB_2881444
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDP12830
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Cadherins are a family of transmembrane glycoproteins that mediate calcium-dependent cell-cell adhesion and play an important role in the maintenance of normal tissue architecture. E-cadherin (epithelial cadherin), also known as CDH1 (cadherin 1) or CAM 120/80, is a classical member of the cadherin superfamily which also include N-, P-, R-, and B-cadherins. E-cadherin is expressed on the cell surface in most epithelial tissues. The extracellular region of E-cadherin establishes calcium-dependent homophilic trans binding, providing specific interaction with adjacent cells, while the cytoplasmic domain is connected to the actin cytoskeleton through the interaction with p120-, α-, β-, and γ-catenin (plakoglobin). E-cadherin is important in the maintenance of the epithelial integrity, and is involved in mechanisms regulating proliferation, differentiation, and survival of epithelial cell. E-cadherin may also play a role in tumorigenesis. It is considered to be an invasion suppressor protein and its loss is an indicator of high tumor aggressiveness.

实验方案

Product Specific Protocols
IF protocol for E-cadherin antibody 60335-1-IgDownload protocol
IHC protocol for E-cadherin antibody 60335-1-IgDownload protocol
WB protocol for E-cadherin antibody 60335-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseIHC

Adv Mater

Glycated ECM Derived Carbon Dots Inhibit Tumor Vasculogenic Mimicry by Disrupting RAGE Nuclear Translocation and Its Interaction With HMGB1

Authors - Shaoting Zheng
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humanIF

Theranostics

FSH induces EMT in ovarian cancer via ALKBH5-regulated Snail m6A demethylation

Authors - Xingyan Xu
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mouseWB,IHC

Theranostics

Vitamin D binding protein (VDBP) hijacks twist1 to inhibit vasculogenic mimicry in hepatocellular carcinoma

Authors - Lu-Ning Qin
View Article
humanWB,IHC

EBioMedicine

PMID: 31594754

Authors - Yiyi Liu
View Article
IHC

J Adv Res

HnRNPR-mediated UPF3B mRNA splicing drives hepatocellular carcinoma metastasis

Authors - Hong Wang
View Article
humanWB

Biomaterials

Urinary exosomes-based Engineered Nanovectors for Homologously Targeted Chemo-Chemodynamic Prostate Cancer Therapy via abrogating IGFR/AKT/NF-kB/IkB signaling.

Authors - Shaojun Pan
View Article
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