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验证数据展示

  • WB analysis of PC-3 using 60335-1-Ig

    WB analysis of PC-3 using 60335-1-Ig

    PC-3, MKN-45, SGC-7901 cells were subjected to SDS PAGE followed by western blot with 60335-1-Ig (E-cadherin Antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • WB analysis of A431 using 60335-1-Ig

    WB analysis of A431 using 60335-1-Ig

    WB result of E-cadherin antibody (60335-1-Ig; 1:5000; incubated at room temperature for 1.5 hours) with sh-Control and sh-E-cadherin transfected A431 cells.

  • WB analysis using 60335-1-Ig

    WB analysis using 60335-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 60335-1-Ig (E-cadherin antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IF Staining of human kidney using 60335-1-Ig

    IF Staining of human kidney using 60335-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed human kidney tissue using E-cadherin antibody (60335-1-Ig, Clone: 6B11F11 ) at dilution of 1:300 and CoraLite®594-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), Podocalyxin antibody(18150-1-AP, green). DNA was stained by DAPI (blue).

  • IF Staining of human breast cancer using 60335-1-Ig

    IF Staining of human breast cancer using 60335-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed human breast cancer tissue using E-cadherin antibody (60335-1-Ig, Clone: 6B11F11 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

  • IF Staining of MCF-7 using 60335-1-Ig

    IF Staining of MCF-7 using 60335-1-Ig

    Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using E-cadherin antibody (60335-1-Ig, Clone: 6B11F11 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

  • IHC staining of human breast cancer using 60335-1-Ig

    IHC staining of human breast cancer using 60335-1-Ig

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 60335-1-Ig (E-cadherin antibody) at dilution of 1:2000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with E-cadherin antibody included
  • IHC staining of human breast cancer using 60335-1-Ig

    IHC staining of human breast cancer using 60335-1-Ig

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 60335-1-Ig (E-cadherin antibody) at dilution of 1:2000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with E-cadherin antibody included
  • IHC staining of human liver using 60335-1-Ig

    IHC staining of human liver using 60335-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 60335-1-Ig (E-cadherin antibody) at dilution of 1:3000. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). The staining was performed on an autostainer.

  • IHC staining of human ovary cancer using 60335-1-Ig

    IHC staining of human ovary cancer using 60335-1-Ig

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 60335-1-Ig (E-cadherin antibody) at dilution of 1:3000. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). The staining was performed on an autostainer.

  • IHC staining of human breast cancer using 60335-1-Ig

    IHC staining of human breast cancer using 60335-1-Ig

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 60335-1-Ig (E-cadherin antibody) at dilution of 1:3000. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). The staining was performed on an autostainer.

  • IHC staining of human colorectal cancer using 60335-1-Ig

    IHC staining of human colorectal cancer using 60335-1-Ig

    Immunohistochemical analysis of paraffin-embedded human colorectal cancer tissue slide using 60335-1-Ig (E-cadherin antibody) at dilution of 1:3000. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). The staining was performed on an autostainer.

  • IHC staining of human colorectal cancer using 60335-1-Ig

    IHC staining of human colorectal cancer using 60335-1-Ig

    Immunohistochemical analysis of paraffin-embedded human colorectal cancer tissue slide using 60335-1-Ig (E-cadherin antibody) at dilution of 1:3000. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with E-cadherin antibody included
  • IHC staining of human stomach cancer using 60335-1-Ig

    IHC staining of human stomach cancer using 60335-1-Ig

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 60335-1-Ig (E-cadherin antibody) at dilution of 1:3000. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with E-cadherin antibody included
  • IHC staining of human placenta using 60335-1-Ig

    IHC staining of human placenta using 60335-1-Ig

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 60335-1-Ig (E-cadherin antibody) at dilution of 1:3000. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with E-cadherin antibody included
  • IHC staining of human breast cancer using 60335-1-Ig

    IHC staining of human breast cancer using 60335-1-Ig

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 60335-1-Ig (E-cadherin antibody) at dilution of 1:3000. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with E-cadherin antibody included
  • IHC staining of human breast cancer using 60335-1-Ig

    IHC staining of human breast cancer using 60335-1-Ig

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 60335-1-Ig (E-cadherin antibody) at dilution of 1:3000. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with E-cadherin antibody included
  • IHC staining of human ovary cancer using 60335-1-Ig

    IHC staining of human ovary cancer using 60335-1-Ig

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 60335-1-Ig (E-cadherin antibody) at dilution of 1:3000. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with E-cadherin antibody included
  • IHC staining of human liver using 60335-1-Ig

    IHC staining of human liver using 60335-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 60335-1-Ig (E-cadherin antibody) at dilution of 1:3000. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with E-cadherin antibody included
  • IHC staining of human tonsillitis using 60335-1-Ig

    IHC staining of human tonsillitis using 60335-1-Ig

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 60335-1-Ig (E-cadherin antibody) at dilution of 1:3000. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with E-cadherin antibody included
  • IHC staining of human breast cancer using 60335-1-Ig

    IHC staining of human breast cancer using 60335-1-Ig

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 60335-1-Ig (E-cadherin antibody) at dilution of 1:8000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with E-cadherin antibody included
  • IHC staining of human breast cancer using 60335-1-Ig

    IHC staining of human breast cancer using 60335-1-Ig

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 60335-1-Ig (E-cadherin antibody) at dilution of 1:10000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with E-cadherin antibody included
  • IHC staining of human liver cancer using 60335-1-Ig

    IHC staining of human liver cancer using 60335-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 60335-1-Ig (E-cadherin antibody) at dilution of 1:10000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with E-cadherin antibody included
  • IHC staining of rat colon using 60335-1-Ig

    IHC staining of rat colon using 60335-1-Ig

    Immunohistochemical analysis of paraffin-embedded rat colon tissue slide using 60335-1-Ig (E-cadherin antibody) at dilution of 1:10000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with E-cadherin antibody included
  • IHC staining of human colon using 60335-1-Ig

    IHC staining of human colon using 60335-1-Ig

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 60335-1-Ig (E-cadherin antibody) at dilution of 1:10000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with E-cadherin antibody included
  • IHC staining of human appendicitis using 60335-1-Ig

    IHC staining of human appendicitis using 60335-1-Ig

    Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 60335-1-Ig (E-cadherin antibody) at dilution of 1:3000. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with E-cadherin antibody included
  • IF Staining of mouse kidney using 60335-1-Ig

    IF Staining of mouse kidney using 60335-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse kidney tissue using E-cadherin antibody (60335-1-Ig, Clone: 6B11F11 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1), Podocalyxin antibody (87582-2-RR, Clone: 252967A3, Magenta), CoraLite®594 ACE2 antibody (CL594-21115, red). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of mouse kidney using 60335-1-Ig

    IF Staining of mouse kidney using 60335-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse kidney tissue using E-cadherin antibody (60335-1-Ig, Clone: 6B11F11 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1), CoraLite®594 ACE2 antibody (CL594-21115, red), Podocalyxin antibody (87582-2-RR, Clone: 252967A3, Magenta). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of mouse kidney using 60335-1-Ig

    IF Staining of mouse kidney using 60335-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse kidney tissue using E-cadherin antibody (60335-1-Ig, Clone: 6B11F11 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1), CoraLite®594 ACE2 antibody (CL594-21115, red), Podocalyxin antibody (87582-2-RR, Clone: 252967A3, Magenta). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of mouse brain using 60335-1-Ig

    IF Staining of mouse brain using 60335-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed frozen OCT-embedded mouse brain tissue using E-cadherin antibody (60335-1-Ig, Clone: 6B11F11 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1).

  • IF Staining of mouse breast cancer using 60335-1-Ig

    IF Staining of mouse breast cancer using 60335-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed mouse breast cancer using E-cadherin antibody (60335-1-Ig, Clone: 6B11F11 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1).

  • Featured Product
  • KD/KO Validated

E-cadherin Monoclonal antibody

E-cadherin Monoclonal Antibody for WB, IHC, IF/ICC, IF-P, IF-Fro, ELISA
Cat No. 60335-1-Ig

产品说明书

CloneNo. 6B11F11
引用文献(317)

宿主/亚型

Mouse / IgG2b

种属反应性

human, mouse, rat, pig and More (2)

应用

WB, IHC, IF/ICC, IF-P, IF-Fro, ELISA and More (1)

别名

6B11F11, Arc 1, Cadherin 1, Cadherin-1, CAM 120/80

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inPC-3 cells, A431 cells, MCF-7 cells, pig brain tissue, MKN-45 cells, SGC-7901 cells
Positive IHC detected inhuman breast cancer tissue, human colon tissue, rat colon tissue, rat stomach tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inhuman breast cancer tissue, human kidney tissue, mouse kidney tissue
Positive IF-Fro detected inmouse brain tissue
Positive IF/ICC detected inMCF-7 cells, mouse breast cancer, HaCaT cells
Planning an IHC experiment? We recommend our IHCeasy E-cadherin Ready-To-Use IHC Kit. E-cadherin primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488, CoraLite®555 and CoraLite®594 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:8000
Immunohistochemistry (IHC)IHC : 1:1000-1:4000
Immunofluorescence (IF)-PIF-P : 1:200-1:800
Immunofluorescence (IF)-FROIF-FRO : 1:200-1:800
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

60335-1-Ig targets E-cadherin in WB, IHC, IF/ICC, IF-P, IF-Fro, ELISA applications and shows reactivity with human, mouse, rat, pig samples.

经测试应用 WB, IHC, IF/ICC, IF-P, IF-Fro, ELISA Application Description
文献引用应用WB, IHC, IF
经测试反应性 human, mouse, rat, pig
文献引用反应性human, mouse, rat, pig, canine, monkey
免疫原

CatNo: Ag15085

Product name: Recombinant human E-cadherin protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 373-622 aa of BC141838

Sequence: PIFNPTTYKGQVPENEANVVITTLKVTDADAPNTPAWEAVYTILNDDGGQFVVTTNPVNNDGILKTAKGLDFEAKQQYILHVAVTNVVPFEVSLTTSTATVTVDVLDVNEAPIFVPPEKRVEVSEDFGVGQEITSYTAQEPDTFMEQKITYRIWRDTANWLEINPDTGAISTRAELDREDFEHVKNSTYTALIIATDNGSPVATGTGTLLLILSDVNDNAPIPEPRTIFFCERNPKPQVINIIDADLPPI

种属同源性预测
宿主/亚型 Mouse / IgG2b
抗体类别 Monoclonal
产品类型 Antibody
全称 cadherin 1, type 1, E-cadherin (epithelial)
别名 6B11F11, Arc 1, Cadherin 1, Cadherin-1, CAM 120/80
计算分子量 882 aa, 97 kDa
观测分子量 120 kDa
GenBank蛋白编号BC141838
基因名称 E-cadherin
Gene ID (NCBI) 999
RRIDAB_2881444
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDP12830
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Cadherins are a family of transmembrane glycoproteins that mediate calcium-dependent cell-cell adhesion and play an important role in the maintenance of normal tissue architecture. E-cadherin (epithelial cadherin), also known as CDH1 (cadherin 1) or CAM 120/80, is a classical member of the cadherin superfamily which also include N-, P-, R-, and B-cadherins. E-cadherin is expressed on the cell surface in most epithelial tissues. The extracellular region of E-cadherin establishes calcium-dependent homophilic trans binding, providing specific interaction with adjacent cells, while the cytoplasmic domain is connected to the actin cytoskeleton through the interaction with p120-, α-, β-, and γ-catenin (plakoglobin). E-cadherin is important in the maintenance of the epithelial integrity, and is involved in mechanisms regulating proliferation, differentiation, and survival of epithelial cell. E-cadherin may also play a role in tumorigenesis. It is considered to be an invasion suppressor protein and its loss is an indicator of high tumor aggressiveness.

实验方案

Product Specific Protocols
IF protocol for E-cadherin antibody 60335-1-IgDownload protocol
IHC protocol for E-cadherin antibody 60335-1-IgDownload protocol
WB protocol for E-cadherin antibody 60335-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB,IF

Mol Cancer

OLFM4 promotes the progression of intestinal metaplasia through activation of the MYH9/GSK3β/β-catenin pathway

Authors - Hongfa Wei
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mouseIHC

Adv Mater

Glycated ECM Derived Carbon Dots Inhibit Tumor Vasculogenic Mimicry by Disrupting RAGE Nuclear Translocation and Its Interaction With HMGB1

Authors - Shaoting Zheng
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WB

Cancer Res

SRSF9 Forms Phase-separated Condensates to Promote Ovarian Cancer Progression by Inducing RNA Alternative Splicing that is Inhibited by m6A Modification

Authors - Xinzhao Zuo
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humanWB,IF

Cancer Res

Single-Cell and Spatial Transcriptome Profiling Identifies the Transcription Factor BHLHE40 as a Driver of EMT in Metastatic Colorectal Cancer

Authors - Sheng Yang
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Nat Commun

Self-assembly of hybrid 3D cultures by integrating living and synthetic cells.

Authors - Nils Piernitzki
View Article

Nat Commun

Choline metabolism drives metastasis in BRCA1-deficient ovarian cancers by activating FAM3C.

Authors - Cong Wang
View Article
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