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Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis using 84646-4-RR (same clone as 84646-4-PBS)

    WB analysis using 84646-4-RR (same clone as 84646-4-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 84646-4-RR (E-Cadherin antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84646-4-PBS in a different storage buffer formulation.

  • IHC staining of rat colon using 84646-4-RR (same clone as 84646-4-PBS)

    IHC staining of rat colon using 84646-4-RR (same clone as 84646-4-PBS)

    Immunohistochemical analysis of paraffin-embedded rat colon tissue slide using 84646-4-RR (E-Cadherin antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84646-4-PBS in a different storage buffer formulation.

  • IHC staining of rat colon using 84646-4-RR (same clone as 84646-4-PBS)

    IHC staining of rat colon using 84646-4-RR (same clone as 84646-4-PBS)

    Immunohistochemical analysis of paraffin-embedded rat colon tissue slide using 84646-4-RR (E-Cadherin antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84646-4-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 84646-4-RR

    Affinity and Kinetic Characterization of 84646-4-RR

    Biolayer interferometry (BLl) kinetic assays of 84646-4-RR against Rat E-Cadherin were performed. The affinity constant is 1.38 nM.

Rat E-Cadherin Recombinant antibody, PBS Only

E-Cadherin Uni-rAbTM Recombinant Antibody for WB, IHC, Indirect ELISA
Cat No. 84646-4-PBS

产品说明书

CloneNo. 242018C10

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, Indirect ELISA

别名

242018C10

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

84646-4-PBS targets E-Cadherin in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 Recombinant protein 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 cadherin 1
别名 242018C10
计算分子量99 kDa
观测分子量130 kDa
GenBank蛋白编号NM_031334.1
基因名称 Cdh1
Gene ID (NCBI) 83502
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDQ9R0T4
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Cadherins are a family of transmembrane glycoproteins that mediate calcium-dependent cell-cell adhesion and play an important role in the maintenance of normal tissue architecture. E-cadherin (epithelial cadherin), also known as CDH1 (cadherin 1) or CAM 120/80, is a classical member of the cadherin superfamily which also include N-, P-, R-, and B-cadherins. E-cadherin is expressed on the cell surface in most epithelial tissues. The extracellular region of E-cadherin establishes calcium-dependent homophilic trans binding, providing specific interaction with adjacent cells, while the cytoplasmic domain is connected to the actin cytoskeleton through the interaction with p120-, α-, β-, and γ-catenin (plakoglobin). E-cadherin is important in the maintenance of the epithelial integrity, and is involved in mechanisms regulating proliferation, differentiation, and survival of epithelial cell. E-cadherin may also play a role in tumorigenesis. It is considered to be an invasion suppressor protein and its loss is an indicator of high tumor aggressiveness.