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验证数据展示

  • IF Staining of Jurkat using 85025-6-RR (same clone as 85025-6-PBS)

    IF Staining of Jurkat using 85025-6-RR (same clone as 85025-6-PBS)

    Immunofluorescent analysis of (4% PFA) fixed Jurkat cells using Dysadherin antibody (85025-6-RR, Clone: 252907A7 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 85025-6-PBS in a different storage buffer formulation.

  • WB analysis using 85025-6-RR (same clone as 85025-6-PBS)

    WB analysis using 85025-6-RR (same clone as 85025-6-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 85025-6-RR (FXYD5/Dysadherin antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85025-6-PBS in a different storage buffer formulation.

  • Affinity of 85025-6-RR

    Affinity of 85025-6-RR

    Biolayer interferometry (BLl) kinetic assays of 85025-6-RR against Human Dysadherin were performed. The affinity constant is 13.5 nM.

Dysadherin Recombinant monoclonal antibody, PBS Only

Dysadherin Uni-rAb® Recombinant Antibody for WB, IF/ICC, Indirect ELISA
Cat No. 85025-6-PBS

产品说明书

CloneNo. 252907A7

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IF/ICC, Indirect ELISA

别名

FXYD5, DYSAD, FXYD domain-containing ion transport regulator 5, HSPC113, IWU 1

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy FXYD5 Ready-To-Use IHC Kit. FXYD5 primary antibody included.

产品信息

85025-6-PBS targets Dysadherin in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, IF/ICC, Indirect ELISA Application Description
经测试反应性 human
免疫原

CatNo: Eg5715

Product name: Recombinant human FXYD5/Dysadherin protein

Source: mammalian cells-derived, V37

Tag: C-rFc

Domain: 22-145 aa of NM_001164605.2

Sequence: QTLKDTTSSSSADSTIMDIQVPTRAPDAVYTELQPTSPTPTWPADETPQPQTQTQQLEGTDGPLVTDPETHKSTKAAHPTDDTTTLSERPSPSTDVQTDPQTLKPSGFHEDDPFFYDEHTLRKR

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 FXYD domain containing ion transport regulator 5
别名 FXYD5, DYSAD, FXYD domain-containing ion transport regulator 5, HSPC113, IWU 1
计算分子量19 kDa
观测分子量35-55 kDa
GenBank蛋白编号NM_001164605.2
基因名称 Dysadherin
Gene ID (NCBI) 53827
RRIDAB_3743948
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDQ96DB9
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Dysadherin (also known as FXYD5) is a cancer-related transmembrane glycoprotein, belonging to FXYD family. It is overexpressed on the surface of many tumor cells, and promotes the movement, migration and metastasis of tumor cells by reducing E-cadherin(E- cadherin) dependent intercellular adhesion. Its main function also includes regulating the activity of na, k-ATPase. It is also a typical "abnormal migration" protein-its SDS-PAGE apparent molecular weight (35-55 kDa) is much higher than the theoretical calculation value (~20 kDa). This difference is mainly caused by extensive O- glycosylation modification, which is particularly important in cancer research, because tumor cells often show a higher degree of glycosylation (50-55 kDa), which may be related to their metastasis promoting function(PMID: 17442482;41535258).

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