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Drosha Polyclonal antibody

Drosha Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Conjugate

Unconjugated

Cat No : 55001-1-AP

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Synonyms

DROSHA, ETOHI2, HSA242976, p241, RANSE3L, Ribonuclease 3, Ribonuclease III, ribonuclease type III, nuclear, RN3, RNase III, RNASE3L, RNASEN



经过测试的应用

Positive WB detected inHEK-293 cells, HeLa cells, HepG2 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

55001-1-AP targets Drosha in WB, ELISA applications and shows reactivity with human samples.

Tested Applications WB, ELISA
Cited Applications WB
Tested Reactivity human
Cited Reactivityhuman
Immunogen Peptide 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name ribonuclease type III, nuclear
Synonyms DROSHA, ETOHI2, HSA242976, p241, RANSE3L, Ribonuclease 3, Ribonuclease III, ribonuclease type III, nuclear, RN3, RNase III, RNASE3L, RNASEN
Calculated Molecular Weight 159 kDa
Observed Molecular Weight 90 kDa, 151 kDa
GenBank Accession NumberNM_013235
Gene Symbol RNASEN
Gene ID (NCBI) 29102
RRIDAB_10859254
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9NRR4
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

DROSHA, also named as RN3, RNASE3L, P241 and RNASEN, is double-stranded (ds) RNA-specific endoribonuclease that is involved in the initial step of microRNA (miRNA) biogenesis. It is a component of the microprocessor complex that is required to process primary miRNA transcripts (pri-miRNAs) to release precursor miRNA (pre-miRNA) in the nucleus. Within the microprocessor complex, RNASEN/DROSHA cleaves the 3' and 5' strands of a stem-loop in pri-miRNAs (processing center 11 bp from the dsRNA-ssRNA junction) to release hairpin-shaped pre-miRNAs that are subsequently cut by the cytoplasmic DICER to generate mature miRNAs. RNASEN is involved also in pre-rRNA processing. Cleaves double-strand RNA and does not cleave single-strand RNA. And it is involved in the formation of GW bodies. The antibody is specific to DROSHA. DROSHA/RNASEN has some transcription forms with MW 160kd, 151kd, 138kd and 90kd.

实验方案

Product Specific Protocols
WB protocol for Drosha antibody 55001-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Sci Rep

The Fragment HMGA2-sh-3p20 from HMGA2 mRNA 3'UTR Promotes the Growth of Hepatoma Cells by Upregulating HMGA2.

Authors - Yuan Wang
humanWB

Am J Physiol Cell Physiol

Hydrogen sulfide rescues high glucose-induced migration dysfunction in HUVECs by upregulating miR-126-3p.

Authors - Wen-Long Xue
humanWB

J Gen Virol

HBV induced the discharge of intrinsic antiviral miRNAs in HBV-replicating hepatocytes via extracellular vesicles to facilitate its replication.

Authors - Qiaofang Chu
  • KD Validated

Acta Pharmacol Sin

Post-transcriptional modulation of protein phosphatase PPP2CA and tumor suppressor PTEN by endogenous siRNA cleaved from hairpin within PTEN mRNA 3'UTR in human liver cells.

Authors - Yu-En Gao
humanWB

Cell Death Dis

FoxP3-miR-150-5p/3p suppresses ovarian tumorigenesis via an IGF1R/IRS1 pathway feedback loop.

Authors - Qinkai Zhang
humanWB

J Biol Chem

Zinc-finger protein YY1 suppresses tumor growth of human nasopharyngeal carcinoma by inactivating c-Myc-mediated microRNA-141 transcription.

Authors - Mengna Li