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验证数据展示

  • WB analysis of HepG2 using 25318-1-AP (same clone as 25318-1-PBS)

    WB analysis of HepG2 using 25318-1-AP (same clone as 25318-1-PBS)

    WB result of Desmoplakin antibody (25318-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Desmoplakin transfected HepG2 cells. This data was developed using the same antibody clone with 25318-1-PBS in a different storage buffer formulation.

  • WB analysis using 25318-1-AP (same clone as 25318-1-PBS)

    WB analysis using 25318-1-AP (same clone as 25318-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 25318-1-AP (Desmoplakin antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 25318-1-PBS in a different storage buffer formulation.

  • IF Staining of A431 using 25318-1-AP (same clone as 25318-1-PBS)

    IF Staining of A431 using 25318-1-AP (same clone as 25318-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using Desmoplakin antibody (25318-1-AP) at dilution of 1:1500 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), Beta Actin antibody (66009-1-Ig, Clone: 2D4H5, red). This data was developed using the same antibody clone with 25318-1-PBS in a different storage buffer formulation.

  • IHC staining of rat heart using 25318-1-PBS

    IHC staining of rat heart using 25318-1-PBS

    Immunohistochemical analysis of paraffin-embedded rat heart tissue slide using 25318-1-AP (Desmoplakin antibody) at dilution of 1:5000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of rat heart using 25318-1-PBS

    IHC staining of rat heart using 25318-1-PBS

    Immunohistochemical analysis of paraffin-embedded rat heart tissue slide using 25318-1-AP (Desmoplakin antibody) at dilution of 1:5000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse heart using 25318-1-PBS

    IHC staining of mouse heart using 25318-1-PBS

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 25318-1-AP (Desmoplakin antibody) at dilution of 1:5000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse heart using 25318-1-PBS

    IHC staining of mouse heart using 25318-1-PBS

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 25318-1-AP (Desmoplakin antibody) at dilution of 1:5000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IP experiment of BxPC-3 using 25318-1-AP (same clone as 25318-1-PBS)

    IP experiment of BxPC-3 using 25318-1-AP (same clone as 25318-1-PBS)

    IP result of anti-Desmoplakin (IP:25318-1-AP, 4ug; Detection:25318-1-AP 1:10000) with BxPC-3 cells lysate 2080 ug. This data was developed using the same antibody clone with 25318-1-PBS in a different storage buffer formulation.

  • IHC staining of human bowen disease using 25318-1-AP (same clone as 25318-1-PBS)

    IHC staining of human bowen disease using 25318-1-AP (same clone as 25318-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human bowen disease slide using 25318-1-AP (Desmoplakin antibody) at dilution of 1:1600 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 25318-1-PBS in a different storage buffer formulation.

  • IHC staining of human bowen disease using 25318-1-AP (same clone as 25318-1-PBS)

    IHC staining of human bowen disease using 25318-1-AP (same clone as 25318-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human bowen disease slide using 25318-1-AP (Desmoplakin antibody) at dilution of 1:1600 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 25318-1-PBS in a different storage buffer formulation.

  • IHC staining of human colon using 25318-1-AP (same clone as 25318-1-PBS)

    IHC staining of human colon using 25318-1-AP (same clone as 25318-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 25318-1-AP (Desmoplakin antibody) at dilution of 1:1600 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 25318-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse heart using 25318-1-AP (same clone as 25318-1-PBS)

    IHC staining of mouse heart using 25318-1-AP (same clone as 25318-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 25318-1-AP (Desmoplakin antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 25318-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse heart using 25318-1-AP (same clone as 25318-1-PBS)

    IHC staining of mouse heart using 25318-1-AP (same clone as 25318-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 25318-1-AP (Desmoplakin antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 25318-1-PBS in a different storage buffer formulation.

  • FC experiment of A431 using 25318-1-AP (same clone as 25318-1-PBS)

    FC experiment of A431 using 25318-1-AP (same clone as 25318-1-PBS)

    1X10^6 A431 cells were intracellularly stained with 0.4 ug Anti-Human Desmoplakin (25318-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer. This data was developed using the same antibody clone with 25318-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

Desmoplakin Polyclonal antibody, PBS Only

Desmoplakin Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA
Cat No. 25318-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, FC (Intra), IP, ELISA

别名

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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产品信息

25318-1-PBS targets Desmoplakin in WB, IHC, IF/ICC, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, FC (Intra), IP, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag18065

Product name: Recombinant human DSP protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-350 aa of BC140802

Sequence: MSCNGGSHPRINTLGRMIRAESGPDLRYEVTSGGGGTSRMYYSRRGVITDQNSDGYCQTGTMSRHQNQNTIQELLQNCSDCLMRAELIVQPELKYGDGIQLTRSRELDECFAQANDQMEILDSLIREMRQMGQPCDAYQKRLLQLQEQMRALYKAISVPRVRRASSKGGGGYTCQSGSGWDEFTKHVTSECLGWMRQQRAEMDMVAWGVDLASVEQHINSHRGIHNSIGDYRWQLDKIKADLREKSAIYQLEEEYENLLKASFERMDHLRQLQNIIQATSREIMWINDCEEEELLYDWSDKNTNIAQKQEAFSIRMSQLEVKEKELNKLKQESDQLVLNQHPASDKIEAY

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 desmoplakin
别名
计算分子量 2871 aa, 332 kDa
观测分子量 240-330 kDa
GenBank蛋白编号BC140802
基因名称 DSP
Gene ID (NCBI) 1832
RRIDAB_2880028
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDP15924
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

The desmoplakin (DP) is the most abundant desmosomal proteins and is located at the cytoplasmic portion of the desmosomes which are specialized cell-cell adhesion structures abundant in tissues such as muscle and epidermis that are subjected to mechanical stress. Desmoplakin 1 and 2 (DP 1 and 2) are two splice variants sharing common C-terminal and N-terminal globular head and tail domains, but differ in the length of the rod domain that links them. This antibody detects both DP1 (280-330 kDa) and DP2 (240-260 kDa). (PMID: 16467215, 11781569)

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