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Desmin Monoclonal antibody, PBS Only

Desmin Monoclonal Antibody for WB,Indirect ELISA,IHC,IF

Host / Isotype

Mouse / IgG1

Reactivity

Human, pig, mouse

Applications

WB,Indirect ELISA,IHC,IF

Conjugate

Unconjugated

CloneNo.

2B1A11

Cat No : 67793-1-PBS

Print datasheet

Synonyms

Cell and organelle markers, CMD1I, CSM1, CSM2, Cytoskeleton Marker, DES, desmin, FLJ12025, FLJ39719, FLJ41013, FLJ41793

验证数据展示

  • IHC staining of human colon using 67793-1-Ig (same clone as 67793-1-PBS)

    IHC staining of human colon using 67793-1-Ig (same clone as 67793-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 67793-1-Ig (Desmin antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67793-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with Desmin antibody included
  • IF Staining of human colon using 67793-1-Ig (same clone as 67793-1-PBS)

    IF Staining of human colon using 67793-1-Ig (same clone as 67793-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human colon tissue using Desmin antibody (67793-1-Ig, Clone: 2B1A11 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). DAPI (blue). This data was developed using the same antibody clone with 67793-1-PBS in a different storage buffer formulation.

  • WB analysis using 67793-1-Ig (same clone as 67793-1-PBS)

    WB analysis using 67793-1-Ig (same clone as 67793-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67793-1-Ig (Desmin antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 67793-1-PBS in a different storage buffer formulation.

  • IF Staining of human appendicitis using 67793-1-Ig (same clone as 67793-1-PBS)

    IF Staining of human appendicitis using 67793-1-Ig (same clone as 67793-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human appendicitis tissue using Desmin antibody (67793-1-Ig, Clone: 2B1A11 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67793-1-PBS in a different storage buffer formulation.

  • IF Staining of mouse skeletal muscle using 67793-1-Ig (same clone as 67793-1-PBS)

    IF Staining of mouse skeletal muscle using 67793-1-Ig (same clone as 67793-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed mouse skeletal muscle tissue using Desmin antibody (67793-1-Ig, Clone: 2B1A11 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67793-1-PBS in a different storage buffer formulation.

Planning an IHC experiment? We recommend our IHCeasy Desmin Ready-To-Use IHC Kit. Desmin primary antibody included.

产品信息

67793-1-PBS targets Desmin in WB, IF, IHC, Indirect ELISA applications and shows reactivity with Human, pig, mouse samples.

Tested Applications WB,Indirect ELISA,IHC,IF
Tested Reactivity Human, pig, mouse
Immunogen Desmin fusion protein Ag9675 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name desmin
Synonyms Cell and organelle markers, CMD1I, CSM1, CSM2, Cytoskeleton Marker, DES, desmin, FLJ12025, FLJ39719, FLJ41013, FLJ41793
Calculated Molecular Weight 470 aa, 54 kDa
Observed Molecular Weight52 kDa
GenBank Accession NumberBC032116
Gene Symbol DES
Gene ID (NCBI) 1674
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP17661
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

背景介绍

Desmin is the main intermediate filament protein in skeletal and cardiac muscle cells and is essential for both the structural integrity and the survival of muscle cells. As an abundant muscle-specific protein, desmin has been widely used as a marker of muscle derived tumors. Anti-desmin is also valuable in the differential diagnosis of tumors of uncertain origin.