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Dermatopontin Monoclonal antibody

Dermatopontin Monoclonal Antibody for IF/ICC, WB, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

human, rat

Applications

IF/ICC, WB, ELISA

Conjugate

Unconjugated

CloneNo.

4B1A12

Cat No : 66348-1-Ig

Print datasheet

Synonyms

dermatopontin, DPT, TRAMP

验证数据展示

  • WB analysis of rat skin using 66348-1-Ig

    WB analysis of rat skin using 66348-1-Ig

    rat skin tissue were subjected to SDS PAGE followed by western blot with 66348-1-Ig (Dermatopontin antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IF Staining of SH-SY5Y using 66348-1-Ig

    IF Staining of SH-SY5Y using 66348-1-Ig

    Immunofluorescent analysis of (-20°C Ethanol) fixed SH-SY5Y cells using Dermatopontin antibody (66348-1-Ig, Clone: 4B1A12 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

经过测试的应用

Positive WB detected inrat skin tissue, human testis tissue
Positive IF detected inSH-SY5Y cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunofluorescence (IF)IF : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

66348-1-Ig targets Dermatopontin in WB, IF, ELISA applications and shows reactivity with human, rat samples.

Tested Applications IF/ICC, WB, ELISA
Tested Reactivity human, rat
Immunogen Dermatopontin fusion protein Ag6200 种属同源性预测
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Full Name dermatopontin
Synonyms dermatopontin, DPT, TRAMP
Calculated Molecular Weight 22 kDa
Observed Molecular Weight 26 kDa
GenBank Accession NumberBC033736
Gene Symbol DPT
Gene ID (NCBI) 1805
RRIDAB_2881728
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ07507
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

DPT (dermatopontin) is an extracellular matrix protein belonging to the dermatopontin family. Skin appears to be the richest source of dermatopontin, comprising about 15 mg/kg of the wet weight. Expression of dermatopontin is not limited to connective tissue, as investigations show that dermatopontin mRNA is expressed in cultured fibroblasts, muscle, heart, pancreas, and lung. Dermatopontin comprises a considerable proportion of the noncollagenous extracellular matrix proteins. It is involved in promotion of cellular adhesion, extracellular matrix assembly, wound healing, and positive modification of the inhibition activity of TGF-beta1.

实验方案

Product Specific Protocols
WB protocol for Dermatopontin antibody 66348-1-IgDownload protocol
IF protocol for Dermatopontin antibody 66348-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols