验证数据展示
经过测试的应用
| Positive WB detected in | HEK-293 cells, HEK-293, TF-1, K-562 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
87728-1-RR targets Darc in WB, ELISA applications and shows reactivity with human, mouse samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | human, mouse |
| 免疫原 |
Peptide 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | Duffy blood group, chemokine receptor |
| 别名 | Ackr1, Atypical chemokine receptor 1, CD234, Dfy, Duffy antigen/chemokine receptor |
| 观测分子量 | 38 kDa, 43 kDa |
| GenBank蛋白编号 | BC005583 |
| 基因名称 | Darc |
| Gene ID (NCBI) | 13349 |
| RRID | AB_3745721 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | Q9QUI6 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Mouse Darc (Ackr1) is an atypical chemokine receptor expressed predominantly on erythrocytes and endothelial cells, where it binds diverse inflammatory chemokines without classical GPCR signaling. By functioning as a chemokine reservoir and transporter, Ackr1 regulates chemokine gradients and leukocyte trafficking during inflammation. Studies in Ackr1-deficient mice highlight its role in immune homeostasis and neutrophil recruitment, establishing Darc as an important regulator of systemic chemokine availability and inflammatory responses.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for Darc antibody 87728-1-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
