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验证数据展示

  • IP experiment of HEK-293T using 33563-1-AP (same clone as 33563-1-PBS)

    IP experiment of HEK-293T using 33563-1-AP (same clone as 33563-1-PBS)

    IP result of anti-DZIP3 (IP:33563-1-AP, 4ug; Detection:33563-1-AP 1:1000) with HEK-293T cells lysate 2080 ug. This data was developed using the same antibody clone with 33563-1-PBS in a different storage buffer formulation.

  • WB analysis using 33563-1-AP (same clone as 33563-1-PBS)

    WB analysis using 33563-1-AP (same clone as 33563-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 33563-1-AP (DZIP3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 33563-1-PBS in a different storage buffer formulation.

  • IHC staining of human kidney using 33563-1-AP (same clone as 33563-1-PBS)

    IHC staining of human kidney using 33563-1-AP (same clone as 33563-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 33563-1-AP (DZIP3 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 33563-1-PBS in a different storage buffer formulation.

  • IHC staining of human kidney using 33563-1-AP (same clone as 33563-1-PBS)

    IHC staining of human kidney using 33563-1-AP (same clone as 33563-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 33563-1-AP (DZIP3 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 33563-1-PBS in a different storage buffer formulation.

  • IF Staining of HEK-293 using 33563-1-AP (same clone as 33563-1-PBS)

    IF Staining of HEK-293 using 33563-1-AP (same clone as 33563-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using DZIP3 antibody (33563-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 33563-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 33563-1-AP (same clone as 33563-1-PBS)

    IF Staining of HeLa using 33563-1-AP (same clone as 33563-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using DZIP3 antibody (33563-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 33563-1-PBS in a different storage buffer formulation.

DZIP3 Polyclonal antibody, PBS Only

DZIP3 Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA
Cat No. 33563-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, IF/ICC, IP, Indirect ELISA

别名

DAZ-interacting protein 3, E3 ubiquitin-protein ligase DZIP3, EC:2.3.2.27, hRUL138, KIAA0675

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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产品信息

33563-1-PBS targets DZIP3 in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF/ICC, IP, Indirect ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag35815

Product name: Recombinant human DZIP3 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 631-862 aa of BC063882

Sequence: AEINKDGTSIPSESSTESLKDLQEVKSKQRKKKKTKNKKNKDSKEDQVPYVVEKEEQLRKEQANPHSVSRLIKDDASDVQEDSAMEDKFYSLDELHILDMIEQGSAGKVTTDYGETEKERLARQRQLYKLHYQCEDFKRQLRTVTFRWQENQMQIKKKDKIIASLNQQVAFGINKVSKLQRQIHAKDNEIKNLKEQLSMKRSQWEMEKHNLESTMKTYVSKLNAETSRALTA

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 DAZ interacting protein 3, zinc finger
别名 DAZ-interacting protein 3, E3 ubiquitin-protein ligase DZIP3, EC:2.3.2.27, hRUL138, KIAA0675
计算分子量 1208 aa, 139 kDa
观测分子量139-150 kDa
GenBank蛋白编号BC063882
基因名称 DZIP3
Gene ID (NCBI) 9666
RRIDAB_3743010
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity Purification
UNIPROT IDQ86Y13
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

DZIP3/hRUL138 is a poorly characterized RNA-binding RING E3-ubiquitin ligase with functions in embryonic development. DZIP3 localizes in both the nucleus and cytoplasm (PMID: 33067265). DZIP3 was mainly reported to be an RNA-binding ligase that causes histone H2A monoubiquitination, which represses transcription. DZIP3 also regulates the differentiation of stem cells (PMID: 32796072).

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