验证数据展示
经过测试的应用
| Positive WB detected in | HEK-293T cells, HeLa cells, NIH/3T3 cells |
| Positive IHC detected in | mouse brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| IF | See 1 publications below |
产品信息
17274-1-AP targets DZIP1L in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse samples.
| 经测试应用 | WB, IHC, ELISA Application Description |
| 文献引用应用 | IF |
| 经测试反应性 | human, mouse |
| 文献引用反应性 | human |
| 免疫原 |
CatNo: Ag11034 Product name: Recombinant human DZIP1L protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 418-767 aa of BC033308 Sequence: KAVDTEEDSPEEEMEDSQDEQHKVLAALRRNPTLLKHFRPILEDTLEEKLESMGIRKDAKGISIQTLRHLESLLRVQREQKARKFSEFLSLRGKLVKEVTSRAKERQENGAAVSQPDGQPSVKSQQSALVTREAQPKTRTLQVALPSTPAEPPPPTRQSHGSHGSSLTQVSAPAPHPGLHGPSSTPPSSGPGMSTPPFSSEEDSEGDRVQRVSLQPPKVPSRMVPRPKDDWDWSDTETSEENAQPPGQGSGTLVQSMVKNLEKQLEAPAKKPAGGVSLFFMPNAGPQRAATPGRKPQLSEDESDLEISSLEDLPLDLDQREKPKPLSRSKLPEKFGTGPQSSGQPRVPAW 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | DAZ interacting protein 1-like |
| 别名 | Cilium assembly protein DZIP1L, DAZ interacting protein 1 like, DAZ-interacting zinc finger protein 1-like, DZIP2, Zinc finger protein DZIP1L |
| 计算分子量 | 767 aa, 87 kDa |
| 观测分子量 | 87 kDa |
| GenBank蛋白编号 | BC033308 |
| 基因名称 | DZIP1L |
| Gene ID (NCBI) | 199221 |
| RRID | AB_3669266 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q8IYY4 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
DZIP1L, or DAZ interacting zinc finger protein 1-like, is a protein implicated in autosomal recessive polycystic kidney disease (ARPKD). DZIP1L has been shown to localize to centrioles and at the distal end of basal bodies, interacting with septin2, a protein that maintains the periciliary diffusion barrier at the ciliary transition zone (PMID: 28530676).
实验方案
| Product Specific Protocols | |
|---|---|
| IHC protocol for DZIP1L antibody 17274-1-AP | Download protocol |
| WB protocol for DZIP1L antibody 17274-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
