验证数据展示
经过测试的应用
| Positive WB detected in | rat skeletal muscle tissue |
| Positive IF/ICC detected in | THP-1 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
18208-1-AP targets DYSFIP1 in WB, IF/ICC, ELISA applications and shows reactivity with human, rat samples.
| 经测试应用 | WB, IF/ICC, ELISA Application Description |
| 经测试反应性 | human, rat |
| 免疫原 |
CatNo: Ag12839 Product name: Recombinant human DYSFIP1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-154 aa of BC093707 Sequence: MPSRTARYARYSPRQRRRRMLADRSVRFPNDVLFLDHIRQGDLEQVGRFIRTRKVSLATIHPSGLAALHEAVLSGNLECVKLLVKYGADIHQRDEAGWTPLHIACSDGYPDIARYLISLGADRDATNDDGDLPSDLIDPDYKELVELFKGTTMD 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | dysferlin interacting protein 1 |
| 别名 | Toonin, Protein phosphatase 1 regulatory subunit 27, PPP1R27, Dysferlin-interacting protein 1 |
| 计算分子量 | 154 aa, 17 kDa |
| 观测分子量 | 17 kDa |
| GenBank蛋白编号 | BC093707 |
| 基因名称 | DYSFIP1 |
| Gene ID (NCBI) | 116729 |
| RRID | AB_3669295 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q86WC6 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
DYSFIP1 is also known as PPP1R27. DYSFIP1 inhibits phosphatase activity of protein phosphatase 1 (PP1) complexes. The molecular weight of DYSFIP1 is 17 kDa.
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for DYSFIP1 antibody 18208-1-AP | Download protocol |
| WB protocol for DYSFIP1 antibody 18208-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
