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验证数据展示

  • WB analysis of mouse lung using 12890-2-AP

    WB analysis of mouse lung using 12890-2-AP

    mouse lung tissue were subjected to SDS PAGE followed by western blot with 12890-2-AP (DYRK4 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • IHC staining of human lymphoma using 12890-2-AP

    IHC staining of human lymphoma using 12890-2-AP

    Immunohistochemical analysis of paraffin-embedded human lymphoma using 12890-2-AP (DYRK4 antibody) at dilution of 1:100 (under 10x lens).

DYRK4 Polyclonal antibody

DYRK4 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 12890-2-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, ELISA

别名

Dual specificity tyrosine-phosphorylation-regulated kinase 4, EC:2.7.12.1

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inmouse lung tissue, human brain tissue, Jurkat cells, mouse testis tissue, U-937 cells
Positive IHC detected inhuman lymphoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2400
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

12890-2-AP targets DYRK4 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag3515

Product name: Recombinant human DYRK4 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 104-400 aa of BC031244

Sequence: YEVLETIGKGSFGQVAKCLDHKNNELVALKIIRNKKRFHQQALMELKILEALRKKDKDNTYNVVHMKDFFYFRNHFCITFELLGINLYELMKNNNFQGFSLSIVRRFTLSVLKCLQMLSVEKIIHCDLKPENIVLYQKGQASVKVIDFGSSCYEHQKVYTYIQSRFYRSPEVILGHPYDVAIDMWSLGCITAELYTGYPLFPGENEVEQLACIMEVLGLPPAGFIQTASRRQTFFDSKGFPKNITNNRGKKRYPDSKDLTMVLKTYDTSFLDFLRRCLVWEPSLRMTPDQALKHAWI

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 dual-specificity tyrosine-(Y)-phosphorylation regulated kinase 4
别名 Dual specificity tyrosine-phosphorylation-regulated kinase 4, EC:2.7.12.1
计算分子量 520 aa, 60 kDa
观测分子量 60 kDa
GenBank蛋白编号BC031244
基因名称 DYRK4
Gene ID (NCBI) 8798
RRIDAB_2094086
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9NR20
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Dual-specificity tyrosine phosphorylation regulated kinase 4(DYRK4), belongs to a conserved family of serine/threonine protein kinases. DYRK4 is thought to function in the regulation of cell differentiation and proliferation, survival, and in development. DYRK4 has 5 isoforms with molecular weight of 59, 72 and 73 kDa.

实验方案

Product Specific Protocols
IHC protocol for DYRK4 antibody 12890-2-APDownload protocol
WB protocol for DYRK4 antibody 12890-2-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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