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验证数据展示

  • WB analysis of mouse lung using 12890-2-AP

    WB analysis of mouse lung using 12890-2-AP

    mouse lung tissue were subjected to SDS PAGE followed by western blot with 12890-2-AP (DYRK4 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • IHC staining of human lymphoma using 12890-2-AP

    IHC staining of human lymphoma using 12890-2-AP

    Immunohistochemical analysis of paraffin-embedded human lymphoma using 12890-2-AP (DYRK4 antibody) at dilution of 1:100 (under 10x lens).

DYRK4 Polyclonal antibody

DYRK4 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 12890-2-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, ELISA

别名

DYRK4

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inmouse lung tissue, human brain tissue, Jurkat cells, mouse testis tissue, U-937 cells
Positive IHC detected inhuman lymphoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2400
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

12890-2-AP targets DYRK4 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 DYRK4 fusion protein Ag3515 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 dual-specificity tyrosine-(Y)-phosphorylation regulated kinase 4
别名 DYRK4
计算分子量 520 aa, 60 kDa
观测分子量 60 kDa
GenBank蛋白编号BC031244
基因名称 DYRK4
Gene ID (NCBI) 8798
RRIDAB_2094086
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9NR20
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Dual-specificity tyrosine phosphorylation regulated kinase 4(DYRK4), belongs to a conserved family of serine/threonine protein kinases. DYRK4 is thought to function in the regulation of cell differentiation and proliferation, survival, and in development. DYRK4 has 5 isoforms with molecular weight of 59, 72 and 73 kDa.

实验方案

Product Specific Protocols
WB protocol for DYRK4 antibody 12890-2-APDownload protocol
IHC protocol for DYRK4 antibody 12890-2-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols