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验证数据展示

  • WB analysis using 21979-1-AP

    WB analysis using 21979-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 21979-1-AP (DYRK2 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • IF Staining of K-562 using 21979-1-AP

    IF Staining of K-562 using 21979-1-AP

    Immunofluorescent analysis of (4% PFA) fixed K-562 cells using DYRK2 antibody (21979-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

DYRK2 Polyclonal antibody

DYRK2 Polyclonal Antibody for WB, IF/ICC, ELISA
Cat No. 21979-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IF/ICC, ELISA

别名

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHL-60 cells, K-562 cells, mouse testis tissue, rat testis tissue
Positive IF/ICC detected inK-562 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:3000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

21979-1-AP targets DYRK2 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 DYRK2 fusion protein Ag13480 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 dual-specificity tyrosine-(Y)-phosphorylation regulated kinase 2
别名
计算分子量 67 kDa
观测分子量67~70 kDa
GenBank蛋白编号BC006375
基因名称 DYRK2
Gene ID (NCBI) 8445
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ92630
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

DYRK (Dual-specificity tyrosine phosphorylation-regulated kinase 2) is the only member of the Dual-specificity tYrosine phosphorylation-Regulated Kinase (DYRK) family that functions as a kinase activity-independent scaffold for an E3 ubiquitin ligase complex (PMID: 33376136). DYRK2 is involved in cell survival, development, differentiation, gene transcription, proteasomal regulation, and microtubule formation. DYRK2 is a key regulator of cell cycle and apoptosis in response to DNA damage via phosphorylation of p53. DYRK2 has two isoforms and is mostly localized in the cytoplasm; however, it accumulates in the nucleus under genotoxic stress conditions (PMID: 31505048, 37886981).

实验方案

Product Specific Protocols
WB protocol for DYRK2 antibody 21979-1-APDownload protocol
IF protocol for DYRK2 antibody 21979-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols