验证数据展示
经过测试的应用
| Positive WB detected in | mouse testis tissue, HeLa cells, human colon tissue, human heart tissue, mouse skeletal muscle tissue |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 1 publications below |
产品信息
12240-1-AP targets DYRK1B in WB, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, ELISA Application Description |
| 文献引用应用 | WB |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human |
| 免疫原 | DYRK1B fusion protein Ag2883 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | dual-specificity tyrosine-(Y)-phosphorylation regulated kinase 1B |
| 别名 | DYRK1B, Minibrain related kinase, MIRK, Mirk protein kinase |
| 计算分子量 | 629 aa, 69 kDa |
| 观测分子量 | 57 kDa |
| GenBank蛋白编号 | BC018751 |
| 基因名称 | DYRK1B |
| Gene ID (NCBI) | 9149 |
| RRID | AB_2093803 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q9Y463 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
DYRK1B(dual-specificity tyrosine-(Y)-phosphorylation regulated kinase 1B) is also named as MIRK. It is a member of the dyrk/minibrain family of serine/threonine kinases that mediate the transition from growth to differentiation in lower eukaryotes and mammals(PMID:15546868). DYRK1B functions as a transcriptional coactivator that is activated by co-expressed MKK3, a MAP kinase that also can activate p38 MAP kinase(PMID:14500717). The DYRK1B splice variants found in each muscle tissue exhibit approximate molecular weights of 70 and 75 kDa(PMID:12902328). It has 3 isoforms produced by alternative splicing of the molecular weight of 65-69 kDa.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for DYRK1B antibody 12240-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
