验证数据展示
经过测试的应用
| Positive WB detected in | HEK-293T cells, L02 cells, mouse brain tissue |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 4 publications below |
| IF | See 2 publications below |
产品信息
15949-1-AP targets DYNC2LI1 in WB, IF, ELISA applications and shows reactivity with human, mouse samples.
| 经测试应用 | WB, ELISA Application Description |
| 文献引用应用 | WB, IF |
| 经测试反应性 | human, mouse |
| 文献引用反应性 | human, mouse |
| 免疫原 | DYNC2LI1 fusion protein Ag8584 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | dynein, cytoplasmic 2, light intermediate chain 1 |
| 别名 | CGI 60, D2LIC, DYNC2LI1, LIC3 |
| 计算分子量 | 352 aa, 40 kDa |
| 观测分子量 | 37-40 kDa, 22 kDa |
| GenBank蛋白编号 | BC006969 |
| 基因名称 | DYNC2LI1 |
| Gene ID (NCBI) | 51626 |
| RRID | AB_2093643 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q8TCX1 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
DYNC2LI1 (D2LIC, LIC3) is a member of cytoplasmic dynein 2 complex that is required for retrograde transport. It is ubiquitously expressed with high expression levels in brain, kidney, lung and testes. In mammalian cells, DYNC2LI1 is localized to centrosomal region Mutations in DYNC2LI1 disrupt cilia function and cause various skeletal ciliopathies. There're some isoforms with MW 40 kDa, 38 kDa, 22 kDa and 16 kDa.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for DYNC2LI1 antibody 15949-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Elife Dynein-2 intermediate chains play crucial but distinct roles in primary cilia formation and function. | ||
Hum Mol Genet Dynll1 is essential for development and promotes endochondral bone formation by regulating intraflagellar Dynein function in primary cilia. | ||
FEBS Lett Recombinant human cytoplasmic dynein heavy chain 1 and 2: observation of dynein-2 motor activity in vitro. | ||
Cilia Mutations in IFT-A satellite core component genes IFT43 and IFT121 produce short rib polydactyly syndrome with distinctive campomelia. | ||