验证数据展示
经过测试的应用
| Positive WB detected in | mouse testis tissue |
| Positive IHC detected in | mouse brain tissue, human placenta tissue, rat kidney tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 3 publications below |
产品信息
18885-1-AP targets DYNC1LI2 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, ELISA Application Description |
| 文献引用应用 | WB |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse |
| 免疫原 | DYNC1LI2 fusion protein Ag13452 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | dynein, cytoplasmic 1, light intermediate chain 2 |
| 别名 | LIC53/55, LIC-2, LIC2, LIC 2, DNCLI2 |
| 计算分子量 | 492 aa, 54 kDa |
| 观测分子量 | 48-50 kDa |
| GenBank蛋白编号 | BC025959 |
| 基因名称 | DYNC1LI2 |
| Gene ID (NCBI) | 1783 |
| RRID | AB_10596910 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | O43237 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Cytoplasmic dynein is a microtubule-associated motor protein. DYNC1LI2 (Cytoplasmic dynein 1 light intermediate chain 2), also known as LIC2, belongs to the dynein light intermediate chain family. DYNC1LI2 acts as one of several non-catalytic accessory components of the cytoplasmic dynein 1 complex that are thought to be involved in linking dynein to cargos and to adapter proteins that regulate dynein function. It may play a role in binding dynein to membranous organelles or chromosomes. Northern blot analysis of rat tissues detected high expression of DYNC1LI2 in skeletal muscle, testis, heart, lung, and brain, and lower expression in liver, kidney, and spleen (PMID: 7738094).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for DYNC1LI2 antibody 18885-1-AP | Download protocol |
| IHC protocol for DYNC1LI2 antibody 18885-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
PLoS Genet Dync1li1 is required for the survival of mammalian cochlear hair cells by regulating the transportation of autophagosomes. | ||
Cell Cycle Tctex1d2 associates with short-rib polydactyly syndrome proteins and is required for ciliogenesis. | ||
