验证数据展示
经过测试的应用
| Positive WB detected in | Jurkat cells, A375 cells, human brain tissue, mouse testis tissue |
| Positive IP detected in | mouse testis tissue, HeLa cells |
| Positive IHC detected in | mouse stomach tissue, mouse cerebellum tissue, rat cerebellum tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | A375 cells |
Planning an IHC experiment? We recommend our IHCeasy DYNC1I2 Ready-To-Use IHC Kit. DYNC1I2 primary antibody included.
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:10-1:100 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
12219-1-AP targets DYNC1I2 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, IF/ICC, IP, ELISA Application Description |
| 文献引用应用 | WB, IP |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human |
| 免疫原 |
CatNo: Ag2873 Product name: Recombinant human DYNC1I2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 304-612 aa of BC015038 Sequence: HCQSAVMSATFAKFHPNLVVGGTYSGQIVLWDNRSNKRTPVQRTPLSAAAHTHPVYCVNVVGTQNAHNLISISTDGKICSWSLDMLSHPQDSMELVHKQSKAVAVTSMSFPVGDVNNFVVGSEEGSVYTACRHGSKAGISEMFEGHQGPITGIHCHAAVGAVDFSHLFVTSSFDWTVKLWTTKNNKPLYSFEDNADYVYDVMWSPTHPALFACVDGMGRLDLWNLNNDTEVPTASISVEGNPALNRVRWTHSGREIAVGDSEGQIVIYDVGEQIAVPRNDEWARFGRTLAEINANRADAEEEAATRIPA 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | dynein, cytoplasmic 1, intermediate chain 2 |
| 别名 | IC2, DNCIC2, DNCI2, DH IC-2, DH IC 2 |
| 计算分子量 | 612 aa, 68 kDa |
| 观测分子量 | 68-72 kDa |
| GenBank蛋白编号 | BC015038 |
| 基因名称 | DYNC1I2 |
| Gene ID (NCBI) | 1781 |
| RRID | AB_2093630 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q13409 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for DYNC1I2 antibody 12219-1-AP | Download protocol |
| IHC protocol for DYNC1I2 antibody 12219-1-AP | Download protocol |
| IF protocol for DYNC1I2 antibody 12219-1-AP | Download protocol |
| IP protocol for DYNC1I2 antibody 12219-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
J Cell Biol Ndel1 suppresses ciliogenesis in proliferating cells by regulating the trichoplein-Aurora A pathway.
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