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  • Featured Product
  • KD/KO Validated

DYNC1I2 Polyclonal antibody

DYNC1I2 Polyclonal Antibody for IF/ICC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF/ICC, IP, WB,ELISA

Conjugate

Unconjugated

Cat No : 12219-1-AP

Print datasheet

Synonyms

DH IC 2, DNCI2, DNCIC2, DYNC1I2, IC2

验证数据展示

  • WB analysis of Jurkat using 12219-1-AP

    WB analysis of Jurkat using 12219-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 12219-1-AP (DYNC1I2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • IP experiment of mouse testis using 12219-1-AP

    IP experiment of mouse testis using 12219-1-AP

    IP result of anti-DYNC1I2 (IP:12219-1-AP, 4ug; Detection:12219-1-AP 1:500) with mouse testis tissue lysate 4000ug.

  • IF Staining of A375 using 12219-1-AP

    IF Staining of A375 using 12219-1-AP

    Immunofluorescent analysis of A375 cells using 12219-1-AP (DYNC1I2 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive WB detected inJurkat cells, A375 cells, human brain tissue, mouse testis tissue
Positive IP detected inmouse testis tissue, HeLa cells
Positive IF detected inA375 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)IF : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

12219-1-AP targets DYNC1I2 in WB, IP, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications IF/ICC, IP, WB,ELISA
Cited ApplicationsWB, IP
Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Immunogen DYNC1I2 fusion protein Ag2873 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name dynein, cytoplasmic 1, intermediate chain 2
Synonyms DH IC 2, DNCI2, DNCIC2, DYNC1I2, IC2
Calculated Molecular Weight 612 aa, 68 kDa
Observed Molecular Weight 68-72 kDa
GenBank Accession NumberBC015038
Gene Symbol DYNC1I2
Gene ID (NCBI) 1781
RRIDAB_2093630
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ13409
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for DYNC1I2 antibody 12219-1-APDownload protocol
IF protocol for DYNC1I2 antibody 12219-1-APDownload protocol
IP protocol for DYNC1I2 antibody 12219-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

J Cell Biol

Ndel1 suppresses ciliogenesis in proliferating cells by regulating the trichoplein-Aurora A pathway.

Authors - Hironori Inaba
  • KD Validated
View Article
humanIP

Development

SPEF2 functions in microtubule-mediated transport in elongating spermatids.

Authors - Mari S Lehti
View Article