验证数据展示
经过测试的应用
| Positive WB detected in | U2OS cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
32123-1-AP targets DYNAP in WB, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | human |
| 免疫原 |
CatNo: Ag37125 Product name: Recombinant human C18orf26 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 135-210 aa of NM_001307955 Sequence: LVNNKGSANSSIVIQLSTNDGECVTVKPGTPSPACPPTMTTTSTVPASTATESTTSTATAATTSTEPITVAPTDHL 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | chromosome 18 open reading frame 26 |
| 别名 | C18orf26, Dynactin-associated protein, Full |
| 计算分子量 | 22 kDa |
| 观测分子量 | 45 kDa |
| GenBank蛋白编号 | NM_001307955 |
| 基因名称 | C18orf26 |
| Gene ID (NCBI) | 284254 |
| RRID | AB_3742530 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity Purification |
| UNIPROT ID | Q8N1N2 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
The dynactin-associated protein (DYNAP), also known as C18orf26, is a transmembrane protein that localises to the Golgi apparatus and the plasma membrane in a microtubule-dependent manner. This protein is expressed in half of all human cancer cell lines. DYNAP expression activates Akt (also known as protein kinase B) by promoting Ser473 phosphorylation, which is required for full activation (PMID: 20978158). In SDS-PAGE, DYNAP migrates anomalously at ~45 kDa, which is much larger than the expected 22.5 kDa based on its amino acid composition. The T/S cluster region is responsible for this (PMID: 35022995).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for DYNAP antibody 32123-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
