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Host
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Species Reactivity
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Species Reactivity
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Conjugate
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Conjugate
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Compatible Species
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Conjugate
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验证数据展示

  • WB analysis using CL750-80010

    WB analysis using CL750-80010

    Various lysates were subjected to SDS PAGE followed by western blot with CL750-80010 (DYKDDDDK tag antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • FC experiment of Transfected HEK-293 using CL750-80010

    FC experiment of Transfected HEK-293 using CL750-80010

    1x10^6 Transfected HEK-293 cells were intracellularly stained with 0.4 ug CoraLite® Plus 750-conjugated DYKDDDDK tag Recombinant antibody (Binds to FLAG® tag epitope) (CL750-80010, Clone:4K14)(red). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

CoraLite® Plus 750-conjugated DYKDDDDK tag Recombinant antibody (Binds to FLAG® tag epitope)

DYKDDDDK tag Recombinant Antibody for WB
Cat No. CL750-80010

产品说明书

CloneNo. 4K14

宿主/亚型

Rabbit / IgG

种属反应性

recombinant protein

应用

WB

别名

DDDDK, Flag tag, Flag, DYKDDDDK, DDDDK tag

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  {{ptg:cur_Conjugation}}
规格: 

-/ -

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经过测试的应用

Positive WB detected inRecombinant protein
Positive FC (Intra) detected inTransfected HEK-293 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:4000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL750-80010 targets DYKDDDDK tag in WB applications and shows reactivity with recombinant protein samples.

经测试应用 WB Application Description
经测试反应性 recombinant protein
免疫原 DYKDDDDK tag fusion protein Ag2329 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 Flag Tag
别名 DDDDK, Flag tag, Flag, DYKDDDDK, DDDDK tag
基因名称 Flag Tag
Gene ID (NCBI) 8
RRIDAB_3086572
偶联类型 CoraLite® Plus 750 Fluorescent Dye
最大激发/发射波长755 nm / 780 nm
形式 Liquid
纯化方式Protein A purification
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

DYKDDDDK Tag (Equivalent To FLAG Antibody From Sigma) with the following sequence DYKDDDDK, is a hydrophilic tag for recombinant protein technology. Tags can be used as a tool to localize gene products in a variety of cell types, study proteins topology, and also help to identify and characterize new, low abundance or poorly immunogenic proteins. Due to its high hydrophilic character, the DYKDDDDK tag is likely to be located on the surface of a fusion protein, which enables the tag to be accessible for antibodies. DYKDDDDK Tag Antibody is generated against 1xDYKDDDDK tag (DYKDDDDK) and can recognize protein containing one or more DDDDK tags, independently on N-terminal, C-terminal or internal regions of the target protein. Anti-FLAG is a registered trademark of Sigma-Aldrich Biotechnology.

实验方案

Product Specific Protocols
WB protocol for CL Plus 750 DYKDDDDK tag antibody CL750-80010Download protocol
Standard Protocols
Click here to view our Standard Protocols