CoraLite® Plus 488-conjugated DYKDDDDK tag Recombinant antibody (Binds to FLAG® tag epitope)
DYKDDDDK tag Recombinant Antibody for IF
Host / Isotype
Rabbit / IgG
Reactivity
recombinant protein
Applications
IF
Conjugate
CoraLite® Plus 488 Fluorescent Dye
CloneNo.
4K14
验证数据展示
经过测试的应用
Positive IF detected in | Transfected HEK-293 cells |
For other applications, we recommend the unconjugated version of this antibody, 80010-1-RR
推荐稀释比
Application | Dilution |
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Immunofluorescence (IF) | IF : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
CL488-80010 targets DYKDDDDK tag in IF applications and shows reactivity with recombinant protein samples.
Tested Applications | IF |
Tested Reactivity | recombinant protein |
Immunogen | DYKDDDDK tag fusion protein Ag2329 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Recombinant |
Type | Antibody |
Full Name | DYKDDDDK tag |
Synonyms | DYKDDDDK, DYKDDDDK tag, Flag tag, Flag-tag |
Gene Symbol | |
Gene ID (NCBI) | |
RRID | AB_2919568 |
Conjugate | CoraLite® Plus 488 Fluorescent Dye |
Excitation/Emission Maxima Wavelengths | 493 nm / 522 nm |
Form | Liquid |
Purification Method | Protein A purification |
Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
Storage Conditions | Store at -20°C. Avoid exposure to light. Aliquoting is unnecessary for -20oC storage. |
背景介绍
DYKDDDDK Tag (Equivalent To FLAG Antibody From Sigma) with the following sequence DYKDDDDK, is a hydrophilic tag for recombinant protein technology. Tags can be used as a tool to localize gene products in a variety of cell types, study proteins topology, and also help to identify and characterize new, low abundance or poorly immunogenic proteins. Due to its high hydrophilic character, the DYKDDDDK tag is likely to be located on the surface of a fusion protein, which enables the tag to be accessible for antibodies. DYKDDDDK Tag Antibody is generated against 1xDYKDDDDK tag (DYKDDDDK) and can recognize protein containing one or more DDDDK tags, independently on N-terminal, C-terminal or internal regions of the target protein. Anti-FLAG is a registered trademark of Sigma-Aldrich Biotechnology.
实验方案
Product Specific Protocols | |
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IF protocol for CL Plus 488 DYKDDDDK tag antibody CL488-80010 | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |