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验证数据展示

  • WB analysis using 66008-4-Ig (same clone as 66008-4-PBS)

    WB analysis using 66008-4-Ig (same clone as 66008-4-PBS)

    HEK-293 and different loading amount of DYKDDDDK tagged TDP-43 transfected HEK-293 cells were subjected to SDS PAGE followed by western blot with 66008-4-Ig (DYKDDDDK tag antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was re-blotted with GAPDH antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 66008-4-PBS in a different storage buffer formulation.

  • IP experiment of Transfected HEK-293 using 66008-4-Ig (same clone as 66008-4-PBS)

    IP experiment of Transfected HEK-293 using 66008-4-Ig (same clone as 66008-4-PBS)

    IP result of anti-DYKDDDDK tag (IP:66008-4-Ig, 5 ug; Detection:80010-1-RR 1:10000) with Transfected HEK-293T cells lysate 250 ug. This data was developed using the same antibody clone with 66008-4-PBS in a different storage buffer formulation.

  • IF Staining of Transfected HEK-293 using 66008-4-Ig (same clone as 66008-4-PBS)

    IF Staining of Transfected HEK-293 using 66008-4-Ig (same clone as 66008-4-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed Transfected HEK-293 cells using DYKDDDDK tag antibody (66008-4-Ig, Clone: 8H6A10 ) at dilution of 1:400 and CoraLite®594-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-3). This data was developed using the same antibody clone with 66008-4-PBS in a different storage buffer formulation.

  • FC experiment of Transfected HEK-293 using 66008-4-Ig (same clone as 66008-4-PBS)

    FC experiment of Transfected HEK-293 using 66008-4-Ig (same clone as 66008-4-PBS)

    1x10^6 Transfected HEK-293 cells were intracellularly stained with 0.25 ug DYKDDDDK tag Monoclonal antibody (Binds to FLAG® tag epitope) (66008-4-Ig, Clone:8H6A10) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1)(red). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer. This data was developed using the same antibody clone with 66008-4-PBS in a different storage buffer formulation.

DYKDDDDK tag Monoclonal antibody, PBS Only (Binds to FLAG® tag epitope)

DYKDDDDK tag Monoclonal Antibody for WB, IF/ICC, FC (Intra), IP, ELISA
Cat No. 66008-4-PBS

产品说明书

CloneNo. 8H6A10
引用文献(4)

宿主/亚型

Mouse / IgG2b

种属反应性

recombinant protein and More (1)

应用

WB, IF/ICC, FC (Intra), IP, ELISA and More (1)

别名

Flag, Flag tag, DDDDK, DDDDK tag, DYKDDDDK

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

66008-4-PBS targets DYKDDDDK tag in WB, IF/ICC, FC (Intra), IP, ChIP, ELISA applications and shows reactivity with recombinant protein samples.

经测试应用 WB, IF/ICC, FC (Intra), IP, ELISA Application Description
文献引用应用WB, ChIP
经测试反应性 recombinant protein
文献引用反应性human
免疫原

Peptide

种属同源性预测
宿主/亚型 Mouse / IgG2b
抗体类别 Monoclonal
产品类型 Antibody
全称 DYKDDDDK tag
别名 Flag, Flag tag, DDDDK, DDDDK tag, DYKDDDDK
基因名称
Gene ID (NCBI)
RRIDAB_2918475
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDFLAGTAG
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

DYKDDDDK Tag ( Equivalent To FLAG Antibody From Sigma ) with the following sequence DYKDDDDK, is a hydrophilic tag for recombinant protein technology. Tags can be used as a tool to localize gene products in a variety of cell types, study proteins topology, and also help to identify and characterize new, low abundance or poorly immunogenic proteins. Due to its high hydrophilic character, the DKYDDDDK tag is likely to be located on the surface of a fusion protein, which enables the tag to be accessible for antibodies. DYKDDDDK Tag Antibody is generated against 1xDYKDDDDK tag (DYKDDDDK) and can recognize protein containing one or more DYKDDDDK tags, independently on N-terminal, C-terminal or internal regions of the target protein. Anti-FLAG is a registered trademark of Sigma-Aldrich Biotechnology.

发表文章

SpeciesApplicationTitle
WB

Cell Mol Biol Lett

DDX17-mediated raptor-dependent mTORC1 activation links autophagy inhibition and metabolic compensation in HCC development.

Authors - Chaoxiang Lv
View Article
humanWB

Cell Death Differ

Targeting the USP7-PRMT6 epigenetic axis overcomes chemoresistance in breast cancer by coordinating H3R2me2a deposition and RNF168 methylation for DNA repair and ferroptosis blockade.

Authors - Tianshu Yang
View Article
WB

Cell Death Dis

TNMD BRICHOS domain attenuates tau pathology and memory deficits in a mouse model of tauopathy.

Authors - Dandan Su
View Article
humanChIP

Cell Death Dis

Loss of FAM60A disrupts Sin3/HDAC control of the Hippo signaling and promotes oncogenic YAP1 activation.

Authors - Mojnu Miah
View Article
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