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验证数据展示

  • WB analysis using 27384-1-AP (same clone as 27384-1-PBS)

    WB analysis using 27384-1-AP (same clone as 27384-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 27384-1-AP (DVL1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 27384-1-PBS in a different storage buffer formulation.

  • IP experiment of HEK-293 using 27384-1-AP (same clone as 27384-1-PBS)

    IP experiment of HEK-293 using 27384-1-AP (same clone as 27384-1-PBS)

    IP result of anti-DVL1 (IP:27384-1-AP, 4ug; Detection:27384-1-AP 1:3000) with HEK-293 cells lysate 1560 ug. This data was developed using the same antibody clone with 27384-1-PBS in a different storage buffer formulation.

  • IHC staining of human breast cancer using 27384-1-AP (same clone as 27384-1-PBS)

    IHC staining of human breast cancer using 27384-1-AP (same clone as 27384-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 27384-1-AP (DVL1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 27384-1-PBS in a different storage buffer formulation.

  • IHC staining of human breast cancer using 27384-1-AP (same clone as 27384-1-PBS)

    IHC staining of human breast cancer using 27384-1-AP (same clone as 27384-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 27384-1-AP (DVL1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 27384-1-PBS in a different storage buffer formulation.

  • IF Staining of MCF-7 using 27384-1-AP (same clone as 27384-1-PBS)

    IF Staining of MCF-7 using 27384-1-AP (same clone as 27384-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using DVL1 antibody (27384-1-AP) at dilution of 1:500 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 27384-1-PBS in a different storage buffer formulation.

  • WB analysis using 27384-1-AP (same clone as 27384-1-PBS)

    WB analysis using 27384-1-AP (same clone as 27384-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 27384-1-AP (DVL1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 27384-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

DVL1 Polyclonal antibody, PBS Only

DVL1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA
Cat No. 27384-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, IF/ICC, IP, Indirect ELISA

别名

Dishevelled 1, Dishevelled-1, DSH homolog 1, DVL, Segment polarity protein dishevelled homolog DVL-1

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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产品信息

27384-1-PBS targets DVL1 in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, IF/ICC, IP, Indirect ELISA Application Description
经测试反应性 human, mouse
免疫原

CatNo: Ag26055

Product name: Recombinant human DVL1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 283-416 aa of BC050454

Sequence: LGQGYPYQYPGPPPCFPPAYQDPGFSYGSGSTGSQQSEGSKSSGSTRSSRRAPGREKERRAAGAGGSGSESDHTAPSGVGSSWRERPAGQLSRGSSPRSQASATAPGLPPPHPTTKAYTVVGGPPGGPPVRELA

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 dishevelled, dsh homolog 1 (Drosophila)
别名 Dishevelled 1, Dishevelled-1, DSH homolog 1, DVL, Segment polarity protein dishevelled homolog DVL-1
计算分子量 75 kDa
观测分子量73-75 kDa
GenBank蛋白编号BC050454
基因名称 DVL1
Gene ID (NCBI) 1855
RRIDAB_2880859
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDO14640
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

DVL1 is one of three DVL homologous proteins (DVL1-3) widely expressed in embryonic development and in the adult central nervous system. Dishevelled proteins are a necessary component of the Wnt and planar cell polarity developmental signaling pathways. Overexpression of DVL1 has been linked to prostate and breast cancers through the Wnt signaling pathway.

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