验证数据展示
经过测试的应用
| Positive WB detected in | U2OS cells, mouse pancreas tissue, HepG2 cells, HuH-7 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
86354-1-RR targets DUSP19 in WB, ELISA applications and shows reactivity with human, mouse samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | human, mouse |
| 免疫原 |
CatNo: Ag3611 Product name: Recombinant human DUSP19 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-217 aa of BC035000 Sequence: MYSLNQEIKAFSRNNLRKQCTRVTTLTGKKIIETWKDARIHVVEEVEPSSGGGCGYVQDLSSDLQVGVIKPWLLLGSQDAAHDLDTLKKNKVTHILNVAYGVENAFLSDFTYKSISILDLPETNILSYFPECFEFIEEAKRKDGVVLVHCNAGVSRAAAIVIGFLMNSEQTSFTSAFSLVKNARPSICPNSGFMEQLRTYQEGKESNKCDRIQENSS 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | dual specificity phosphatase 19 |
| 别名 | Dual specificity phosphatase TS-DSP1, DUSP17, EC:3.1.3.16, LMW DSP3, LMWDSP3 |
| 计算分子量 | 217 aa, 24 kDa |
| 观测分子量 | 26 kDa |
| GenBank蛋白编号 | BC035000 |
| 基因名称 | DUSP19 |
| Gene ID (NCBI) | 142679 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | Q8WTR2 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
DUSP19 (Dual-Specificity Phosphatase 19), also known as SKRP1, LMW-DSP3, or SAPK pathway-regulating phosphatase 1, is a low-molecular-weight member of the dual-specificity phosphatase family. It is encoded on human chromosome 2q32 and expressed in multiple tissues, with the highest levels found in the pancreas and heart. Structurally, DUSP19 contains a conserved catalytic domain that removes phosphate groups from both phospho-tyrosine and phospho-serine/threonine residues, enabling it to negatively or positively regulate MAPK signaling.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for DUSP19 antibody 86354-1-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
