验证数据展示
经过测试的应用
| Positive WB detected in | HaCaT cells, rat skin tissue, human placenta tissue |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
10172-2-AP targets DUSP14 in WB, ELISA applications and shows reactivity with human, rat samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | human, rat |
| 免疫原 |
CatNo: Ag0228 Product name: Recombinant human DUSP14 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-198 aa of BC004448 Sequence: MSSRGHSTLPRTLMAPRMISEGDIGGIAQITSSLFLGRGSVASNRHLLQARGITCIVNATIEIPNFNWPQFEYVKVPLADMPHAPIGLYFDTVADKIHSVSRKHGATLVHCAAGVSRSATLCIAYLMKFHNVCLLEAYNWVKARRPVIRPNVGFWRQLIDYERQLFGKSTVKMVQTPYGIVPDVYEKESRHLMPYWGI 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | dual specificity phosphatase 14 |
| 别名 | Dual specificity protein phosphatase 14, DUSP 14, EC:3.1.3.16, MAP kinase phosphatase 6, Mitogen-activated protein kinase phosphatase 6 |
| 计算分子量 | 22 kDa |
| 观测分子量 | 22 kDa |
| GenBank蛋白编号 | BC004448 |
| 基因名称 | DUSP14 |
| Gene ID (NCBI) | 11072 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | O95147 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
DUSP14, also known as dual specificity phosphatase 14, is a member of the dual-specificity phosphatase (DUSP) family, which belongs to the type I cysteine-based protein-tyrosine phosphatase superfamily. The primary function of DUSP14 is the inactivation of mitogen-activated protein kinases (MAPKs). It achieves this by dephosphorylating both the threonine and tyrosine residues within the conserved Thr-Xaa-Tyr motif found in the activation loop of its substrates. Beyond its role as a MAPK phosphatase, DUSP14 is known to directly interact with and dephosphorylate the TGF-beta-activated kinase 1 (TAK1)-binding protein 1 (TAB1) in T cells, which negatively regulates T-cell receptor signaling and immune responses
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for DUSP14 antibody 10172-2-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
