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验证数据展示

  • WB analysis of HeLa using 15667-1-AP

    WB analysis of HeLa using 15667-1-AP

    WB result of DUSP12 antibody (15667-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-DUSP12 transfected HeLa cells.

  • IHC staining of mouse brain using 15667-1-AP

    IHC staining of mouse brain using 15667-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 15667-1-AP (DUSP12 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse kidney using 15667-1-AP

    IHC staining of mouse kidney using 15667-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 15667-1-AP (DUSP12 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Featured Product
  • KD/KO验证

DUSP12 Polyclonal antibody

DUSP12 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 15667-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, ELISA

别名

DUSP12, YVH1

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inHeLa cells
Positive IHC detected inmouse brain tissue, mouse embryo tissue, mouse kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

15667-1-AP targets DUSP12 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 DUSP12 fusion protein Ag8131 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 dual specificity phosphatase 12
别名 DUSP12, YVH1
计算分子量 370 aa, 43 kDa
观测分子量 35 kDa
GenBank蛋白编号BC006286
基因名称 DUSP12
Gene ID (NCBI) 11266
RRIDAB_3085468
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9UNI6
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

DUSP12 (Dual specificity protein phosphatase 12) belongs to the protein-tyrosine phosphatase family and non-receptor class dual specificity subfamily. DUSP12 is a kind of dual specificity phosphatase. It can dephosphorylate both phosphotyrosine and phosphoserine or phosphothreonine residues and can dephosphorylate glucokinase in vitro (PMID:10446167). In addition, DUSP12 has phosphatase activity with the synthetic substrate 6,8-difluoro-4-methylumbelliferyl phosphate and other in vitro substrates (PMID:24531476). DUSP12 is ubiquitously expressed in spleen, testis, ovary, and peripheral blood leukocytes and lower expression in liver and lung.

实验方案

Product Specific Protocols
WB protocol for DUSP12 antibody 15667-1-APDownload protocol
IHC protocol for DUSP12 antibody 15667-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols