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验证数据展示

  • WB analysis of HeLa using 15667-1-AP

    WB analysis of HeLa using 15667-1-AP

    WB result of DUSP12 antibody (15667-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-DUSP12 transfected HeLa cells.

  • IHC staining of mouse brain using 15667-1-AP

    IHC staining of mouse brain using 15667-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 15667-1-AP (DUSP12 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse kidney using 15667-1-AP

    IHC staining of mouse kidney using 15667-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 15667-1-AP (DUSP12 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Featured Product
  • KD/KO验证

DUSP12 Polyclonal antibody

DUSP12 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 15667-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, ELISA

别名

DUSP12, YVH1

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inHeLa cells
Positive IHC detected inmouse brain tissue, mouse embryo tissue, mouse kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

15667-1-AP targets DUSP12 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag8131

Product name: Recombinant human DUSP12 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-340 aa of BC006286

Sequence: MLEAPGPSDGCELSNPSASRVSCAGQMLEVQPGLYFGGAAAVAEPDHLREAGITAVLTVDSEEPSFKAGPGVEDLWRLFVPALDKPETDLLSHLDRCVAFIGQARAEGRAVLVHCHAGVSRSVAIITAFLMKTDQLPFEKAYEKLQILKPEAKMNEGFEWQLKLYQAMGYEVDTSSAIYKQYRLQKVTEKYPELQNLPQELFAVDPTTVSQGLKDEVLYKCRKCRRSLFRSSSILDHREGSGPIAFAHKRMTPSSMLTTGRQAQCTSYFIEPVQWMESALLGVMDGQLLCPKCSAKLGSFNWYGEQCSCGRWITPAFQIHKNRVDEMKILPVLGSQTGKI

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 dual specificity phosphatase 12
别名 DUSP12, YVH1
计算分子量 370 aa, 43 kDa
观测分子量 35 kDa
GenBank蛋白编号BC006286
基因名称 DUSP12
Gene ID (NCBI) 11266
RRIDAB_3085468
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9UNI6
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

DUSP12 (Dual specificity protein phosphatase 12) belongs to the protein-tyrosine phosphatase family and non-receptor class dual specificity subfamily. DUSP12 is a kind of dual specificity phosphatase. It can dephosphorylate both phosphotyrosine and phosphoserine or phosphothreonine residues and can dephosphorylate glucokinase in vitro (PMID:10446167). In addition, DUSP12 has phosphatase activity with the synthetic substrate 6,8-difluoro-4-methylumbelliferyl phosphate and other in vitro substrates (PMID:24531476). DUSP12 is ubiquitously expressed in spleen, testis, ovary, and peripheral blood leukocytes and lower expression in liver and lung.

实验方案

Product Specific Protocols
WB protocol for DUSP12 antibody 15667-1-APDownload protocol
IHC protocol for DUSP12 antibody 15667-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols