验证数据展示
经过测试的应用
| Positive WB detected in | A431 cells, HeLa cells, K-562 cells |
| Positive IP detected in | K-562 cells |
| Positive IF/ICC detected in | A431 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:12000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:700-1:2800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| KD/KO | See 2 publications below |
| WB | See 10 publications below |
产品信息
10204-2-AP targets DUSP11 in WB, IF/ICC, IP, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, IF/ICC, IP, ELISA Application Description |
| 文献引用应用 | WB |
| 经测试反应性 | human |
| 文献引用反应性 | human |
| 免疫原 |
CatNo: Ag0276 Product name: Recombinant human DUSP11 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-203 aa of BC000346 Sequence: MSQWHHPRSGWGRRRDFSGRSSAKKKGGNHIPERWKDYLPVGQRMPGTRFIAFKVPLQKSFEKKLAPEECFSPLDLFNKIREQNEELGLIIDLTYTQRYYKPEDLPETVPYLKIFTVGHQVPDDETIFKFKHAVNGFLKENKDNDKLIGVHCTHGLNRTGYLICIYLIDVEGVRPDDAIELFNRCRGHCLERQNYIEDLQNGP 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | dual specificity phosphatase 11 (RNA/RNP complex 1-interacting) |
| 别名 | RNA/RNP complex-1-interacting phosphatase, PIR1, EC:3.1.3.-, DUSP 11, Dual specificity protein phosphatase 11 |
| 计算分子量 | 39 kDa |
| 观测分子量 | 39 kDa |
| GenBank蛋白编号 | BC000346 |
| 基因名称 | DUSP11 |
| Gene ID (NCBI) | 8446 |
| RRID | AB_2277484 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | O75319 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
DUSP11, also known as PIR1, is a unique member of atypical DUSPs which could bind directly to RNA and possess RNA 5'-triphosphatase and diphosphatase activities. DUSP11 converts the 5' triphosphate of microRNA precursors to a 5' monophosphate, and regulates cellular noncoding RNAs levels. In addition to a catalysis towards RNA, more evidence showed that DUSP11 could also dephosphorylate proteins.
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for DUSP11 antibody 10204-2-AP | Download protocol |
| IP protocol for DUSP11 antibody 10204-2-AP | Download protocol |
| WB protocol for DUSP11 antibody 10204-2-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Nat Commun RIG-I like receptor sensing of host RNAs facilitates the cell-intrinsic immune response to KSHV infection. | ||
Proc Natl Acad Sci U S A RNA triphosphatase DUSP11 enables exonuclease XRN-mediated restriction of hepatitis C virus.
| ||
Genes Dev DUSP11 activity on triphosphorylated transcripts promotes Argonaute association with noncanonical viral microRNAs and regulates steady-state levels of cellular noncoding RNAs. | ||
Virol Sin HBV precore G1896A mutation promotes growth of hepatocellular carcinoma cells by activating ERK/MAPK pathway
| ||
RNA Biol DUSP11 - an RNA phosphatase that regulates host and viral non-coding RNAs in mammalian cells. |
