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验证数据展示

  • WB analysis using 83265-8-RR (same clone as 83265-8-PBS)

    WB analysis using 83265-8-RR (same clone as 83265-8-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83265-8-RR (DUS2L antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83265-8-PBS in a different storage buffer formulation.

  • FC experiment of HeLa using 83265-8-RR (same clone as 83265-8-PBS)

    FC experiment of HeLa using 83265-8-RR (same clone as 83265-8-PBS)

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug DUS2L Recombinant antibody (83265-8-RR, Clone:240192C6) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Control Antibody (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer. This data was developed using the same antibody clone with 83265-8-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 83265-8-RR

    Affinity and Kinetic Characterization of 83265-8-RR

    Biolayer interferometry (BLl) kinetic assays of 83265-8-RR against Human DUS2L were performed. The affinity constant is 0.321 nM.

DUS2L Recombinant monoclonal antibody, PBS Only

DUS2L Uni-rAb® Recombinant Antibody for WB, ELISA
Cat No. 83265-8-PBS

产品说明书

CloneNo. 240192C6

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, ELISA

别名

240192C6, Dihydrouridine synthase 2, DUS2, EC:1.3.1.91, FLJ20399

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

83265-8-PBS targets DUS2L in WB, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, ELISA Application Description
经测试反应性 human, mouse
免疫原

CatNo: Ag23831

Product name: Recombinant human DUS2L protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 217-493 aa of BC006527

Sequence: SHDHIQQYSDIEDFRQATAASSVMVARAAMWNPSIFLKEGLRPLEEVMQKYIRYAVQYDNHYTNTKYCLCQMLREQLESPQGRLLHAAQSSREICEAFGLGAFYEETTQELDAQQARLSAKTSEQTGEPAEDTSGVIKMAVKFDRRAYPAQITPKMCLLEWCRREKLAQPVYETVQRPLDRLFSSIVTVAEQKYQSTLWDKSKKLAEQAAAIVCLRSQGLPEGRLGEESPSLHKRKREAPDQDPGGPRAQELAQPGDLCKKPFVALGSGEESPLEGW

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 dihydrouridine synthase 2-like, SMM1 homolog (S. cerevisiae)
别名 240192C6, Dihydrouridine synthase 2, DUS2, EC:1.3.1.91, FLJ20399
观测分子量 55 kDa
GenBank蛋白编号BC006527
基因名称 DUS2L
Gene ID (NCBI) 54920
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purfication
UNIPROT IDQ9NX74
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Dihydrouridine synthase 2(DUS2L), is a cytoplasmic protein that catalyzes the conversion of uridine residues to dihydrouridine in the D-loop of tRNA. transfection of DUS2 cDNA into a human lung carcinoma cell line increased dihydrouridine in tRNA, whereas transfection of DUS2 small interfering RNA (siRNA) reduced tRNA-dihydrouridine, confirming that DUS2 has dihydrouridine synthase activity.

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