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验证数据展示

  • WB analysis using 25259-1-AP (same clone as 25259-1-PBS)

    WB analysis using 25259-1-AP (same clone as 25259-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 25259-1-AP (DUPD1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 25259-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse skeletal muscle using 25259-1-AP (same clone as 25259-1-PBS)

    IHC staining of mouse skeletal muscle using 25259-1-AP (same clone as 25259-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 25259-1-AP (DUPD1 / DUSP27 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 25259-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse skeletal muscle using 25259-1-AP (same clone as 25259-1-PBS)

    IHC staining of mouse skeletal muscle using 25259-1-AP (same clone as 25259-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 25259-1-AP (DUPD1 / DUSP27 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 25259-1-PBS in a different storage buffer formulation.

DUPD1 / DUSP27 Polyclonal antibody, PBS Only

DUPD1 / DUSP27 Polyclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 25259-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, Indirect ELISA

别名

DUPD1, Dual specificity phosphatase 29, DUSP27, DUSP29, EC:3.1.3.16, EC:3.1.3.48

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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产品信息

25259-1-PBS targets DUPD1 / DUSP27 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag19658

Product name: Recombinant human DUPD1 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-150 aa of BC137322

Sequence: MTSGEVKTSLKNAYSSAKRLSPKMEEEGEEEDYCTPGAFELERLFWKGSPQYTHVNEVWPKLYIGDEATALDRYRLQKAGFTHVLNAAHGRWNVDTGPDYYRDMDIQYHGVEADDLPTFDLSVFFYPAAAFIDRALSDDHSKILVHCVMG

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 dual specificity phosphatase and pro isomerase domain containing 1
别名 DUPD1, Dual specificity phosphatase 29, DUSP27, DUSP29, EC:3.1.3.16, EC:3.1.3.48
计算分子量 220 aa, 25 kDa
观测分子量27 kDa
GenBank蛋白编号BC137322
基因名称 DUSP27
Gene ID (NCBI) 338599
RRIDAB_3085779
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ68J44
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Dupd1also named as Dusp27, has been categorized as an atypical dual-specificity phosphatase (Dusp) capable of dephosphorylating both tyrosine and serine/threonine residues. It's involved in the modulation of intracellular signaling cascades. In skeletal muscle Dupd1 regulates systemic glucose homeostasis by activating AMPK, which is an energy sensor protein kinase. Dupd1 also affects MAP kinase signaling though modulation of the MAPK1/2 cascade in skeletal muscle promoting muscle cell differentiation, development and atrophy.

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