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验证数据展示

  • WB analysis of PC-3 using 22088-1-AP

    WB analysis of PC-3 using 22088-1-AP

    PC-3 cells were subjected to SDS PAGE followed by western blot with 22088-1-AP (DULLARD Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • IHC staining of human ovary cancer using 22088-1-AP

    IHC staining of human ovary cancer using 22088-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 22088-1-AP (DULLARD antibody) at dilution of 1:100 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of PC-3 using 22088-1-AP

    IF Staining of PC-3 using 22088-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed PC-3 cells using DULLARD antibody (22088-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

DULLARD Polyclonal antibody

DULLARD Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 22088-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, IF/ICC, ELISA

别名

Serine/threonine-protein phosphatase dullard, NET56, HSA011916, EC:3.1.3.16, CTDNEP1

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inPC-3 cells, mouse skeletal muscle tissue
Positive IHC detected inhuman ovary cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inPC-3 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

22088-1-AP targets DULLARD in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
经测试反应性 human, mouse
免疫原

CatNo: Ag17288

Product name: Recombinant human DULLARD protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-244 aa of BC009295

Sequence: MMRTQCLLGLRTFVAFAAKLWSFFIYLLRRQIRTVIQYQTVRYDILPLSPVSRNRLAQVKRKILVLDLDETLIHSHHDGVLRPTVRPGTPPDFILKVVIDKHPVRFFVHKRPHVDFFLEVVSQWYELVVFTASMEIYGSAVADKLDNSRSILKRRYYRQHCTLELGSYIKDLSVVHSDLSSIVILDNSPGAYRSHPDNAIPIKSWFSDPSDTALLNLLPMLDALRFTADVRSVLSRNLHQHRLW

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 dullard homolog (Xenopus laevis)
别名 Serine/threonine-protein phosphatase dullard, NET56, HSA011916, EC:3.1.3.16, CTDNEP1
计算分子量 244 aa, 28 kDa
观测分子量 28 kDa
GenBank蛋白编号BC009295
基因名称 DULLARD
Gene ID (NCBI) 23399
RRIDAB_2878991
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDO95476
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for DULLARD antibody 22088-1-APDownload protocol
IHC protocol for DULLARD antibody 22088-1-APDownload protocol
IF protocol for DULLARD antibody 22088-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols