验证数据展示
经过测试的应用
| Positive WB detected in | SGC-7901 cells |
Planning an IHC experiment? We recommend our IHCeasy DUB3 Ready-To-Use IHC Kit. DUB3 primary antibody included.
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
26143-1-AP targets DUB3 in WB, IHC, IP, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, ELISA Application Description |
| 文献引用应用 | WB, IHC, IP |
| 经测试反应性 | human |
| 文献引用反应性 | human, mouse |
| 免疫原 |
CatNo: Ag23567 Product name: Recombinant human DUB3 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 374-530 aa of BC157073 Sequence: QKSEWERHSESVSRGREPRALGAEDTDRRATQGELKRDHPCLQAPELDEHLVERATQESTLDHWKFLQEQNKTKPEFNVRKVEGTLPPDVLVIHQSKYKCGMKNHHPEQQSSLLNLSSTTPTHQESMNTGTLASLRGRARRSKGKNKHSKRALLVCQ 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | deubiquitinating enzyme 3 |
| 别名 | Deubiquitinating enzyme 17-like protein 2, deubiquitinating enzyme 3, Deubiquitinating protein 3, DUB 3, DUB-3 |
| 观测分子量 | 60 kDa |
| GenBank蛋白编号 | BC157073 |
| 基因名称 | DUB3 |
| Gene ID (NCBI) | 377630 |
| RRID | AB_2880401 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q6R6M4 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for DUB3 antibody 26143-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Phytomedicine Sarmentosin promotes USP17 and regulates Nrf2-mediated mitophagy and cellular oxidative stress to alleviate APAP-induced acute liver failure. | ||
Front Pharmacol Lysophosphatidic Acid-Induced EGFR Transactivation Promotes Gastric Cancer Cell DNA Replication by Stabilizing Geminin in the S Phase. | ||
Breast Cancer Res Treat USP17L2-SIRT7 axis regulates DNA damage repair and chemoresistance in breast cancer cells
| ||
Oncogene C4orf19 inhibits colorectal cancer cell proliferation by competitively binding to Keap1 with TRIM25 via the USP17/Elk-1/CDK6 axis | ||
iScience DUB3 is a MAGEA3 deubiquitinase and a potential therapeutic target in hepatocellular carcinoma | ||
FASEB J Melatonin alleviates hyperoxia-induced lung injury through elevating MSC exosomal miR-18a-5p expression to repress PUM2 signaling |