验证数据展示
经过测试的应用
| Positive WB detected in | mouse kidney tissue |
| Positive IF-P detected in | mouse embryo tissue |
| Positive IF/ICC detected in | HEK-293 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:200-1:1000 |
| Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:10-1:100 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
25222-1-AP targets DTX4 in WB, IF/ICC, IF-P, ELISA applications and shows reactivity with human, mouse samples.
| 经测试应用 | WB, IF/ICC, IF-P, ELISA Application Description |
| 文献引用应用 | WB, IF |
| 经测试反应性 | human, mouse |
| 文献引用反应性 | human, mouse, rat |
| 免疫原 |
CatNo: Ag18415 Product name: Recombinant human DTX4 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 51-213 aa of BC119011 Sequence: LDLIYPMVTGTLPKAQSWPVSPGPATSPPMSPCSCPQCVLVMSVKAAVVNGSTGPLQLPVTRKNMPPPGVVKLPPLPGSGAKPLDSTGTIRGPLKTAPSQVIRRQASSMPTGTTMGSPASPPGPNSKTGRVALATLNRTNLQRLAIAQSRVLIASGVPTVPVK 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | deltex homolog 4 (Drosophila) |
| 别名 | Protein deltex 4, KIAA0937, EC:2.3.2.27, E3 ubiquitin-protein ligase DTX4, Deltex4 |
| 计算分子量 | 619 aa, 67 kDa |
| 观测分子量 | 67-70 kDa |
| GenBank蛋白编号 | BC119011 |
| 基因名称 | DTX4 |
| Gene ID (NCBI) | 23220 |
| RRID | AB_2879970 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q9Y2E6 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
DTX4, also named as KIAA0937, RNF155, is a 619 amino acid protein, which belongs to the Deltex family. DTX4 as a regulator of Notch signaling, a signaling pathway involved in cell-cell communications that regulates a broad spectrum of cell-fate determinations. DTX4 functions as a ubiquitin ligase protein in vivo, mediating 'Lys48'-linked polyubiquitination and promoting degradation of TBK1, targeting to TBK1 requires interaction with NLRP4.
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for DTX4 antibody 25222-1-AP | Download protocol |
| WB protocol for DTX4 antibody 25222-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Cancer Sci Ubiquitination of NOTCH2 by DTX3 suppresses the proliferation and migration of human esophageal carcinoma. | ||
Stem Cells Dev Combined Methylome and Transcriptome Analysis During Rat Hepatic Stellate Cell Activation. | ||
Biochem Biophys Res Commun E3 ubiquitin ligase DTX4 is required for adipogenic differentiation in 3T3-L1 preadipocytes cell line.
| ||
Cell Death Dis Gasdermin D silencing alleviates airway inflammation and remodeling in an ovalbumin-induced asthmatic mouse model |
