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验证数据展示

  • WB analysis of MCF-7 using 11963-1-AP

    WB analysis of MCF-7 using 11963-1-AP

    MCF-7 cells were subjected to SDS PAGE followed by western blot with 11963-1-AP (DTX3L antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • WB analysis of U2OS using 11963-1-AP

    WB analysis of U2OS using 11963-1-AP

    WB result of DTX3L antibody (11963-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-DTX3L transfected U2OS cells.

  • IHC staining of human ovary cancer using 11963-1-AP

    IHC staining of human ovary cancer using 11963-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 11963-1-AP (DTX3L antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with DTX3L antibody included
  • IHC staining of human tonsillitis using 11963-1-AP

    IHC staining of human tonsillitis using 11963-1-AP

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 11963-1-AP (DTX3L antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with DTX3L antibody included
  • IHC staining of mouse lung using 11963-1-AP

    IHC staining of mouse lung using 11963-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 11963-1-AP (DTX3L antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with DTX3L antibody included
  • IHC staining of mouse ovary using 11963-1-AP

    IHC staining of mouse ovary using 11963-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse ovary tissue slide using 11963-1-AP (DTX3L antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with DTX3L antibody included
  • IHC staining of rat kidney using 11963-1-AP

    IHC staining of rat kidney using 11963-1-AP

    Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 11963-1-AP (DTX3L antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with DTX3L antibody included
  • IHC staining of rat lung using 11963-1-AP

    IHC staining of rat lung using 11963-1-AP

    Immunohistochemical analysis of paraffin-embedded rat lung tissue slide using 11963-1-AP (DTX3L antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with DTX3L antibody included
  • IHC staining of rat ovary using 11963-1-AP

    IHC staining of rat ovary using 11963-1-AP

    Immunohistochemical analysis of paraffin-embedded rat ovary tissue slide using 11963-1-AP (DTX3L antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with DTX3L antibody included
  • IHC staining of mouse ovary using 11963-1-AP

    IHC staining of mouse ovary using 11963-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse ovary tissue slide using 11963-1-AP (DTX3L antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with DTX3L antibody included
  • IHC staining of rat ovary using 11963-1-AP

    IHC staining of rat ovary using 11963-1-AP

    Immunohistochemical analysis of paraffin-embedded rat ovary tissue slide using 11963-1-AP (DTX3L antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with DTX3L antibody included
  • IHC staining of human ovary cancer using 11963-1-AP

    IHC staining of human ovary cancer using 11963-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 11963-1-AP (DTX3L antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with DTX3L antibody included
  • KD/KO Validated

DTX3L Polyclonal antibody

DTX3L Polyclonal Antibody for WB, IHC, ELISA
Cat No. 11963-1-AP

产品说明书

引用文献(6)

宿主/亚型

Rabbit / IgG

种属反应性

human and More (1)

应用

WB, IHC, ELISA

别名

BBAP, B-lymphoma- and BAL-associated protein, deltex 3 like (Drosophila), E3 ubiquitin-protein ligase DTX3L, EC:2.3.2.27

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inMCF-7 cells, U2OS cells
Positive IHC detected inhuman ovary cancer tissue, mouse lung tissue, rat kidney tissue, rat ovary tissue, mouse ovary tissue, rat lung tissue, human tonsillitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy DTX3L Ready-To-Use IHC Kit. DTX3L primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

11963-1-AP targets DTX3L in WB, IHC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, ELISA Application Description
文献引用应用WB, IHC
经测试反应性 human
文献引用反应性human, goat
免疫原

CatNo: Ag2568

Product name: Recombinant human DTX3L protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 430-741 aa of BC060509

Sequence: SVHAYASFIDAFQHASCQLMREVLLLKSLGKERKHLHQTKFADDFRKRHPNVHFVLNQESMTLTGLPNHLAKAKQYVLKGGGMSSLAGKKLKEGHETPMDIDSDDSKAASPPLKGSVSSEASELDKKEKGICVICMDTISNKKVLPKCKHEFCAPCINKAMSYKPICPTCQTSYGIQKGNQPEGSMVFTVSRDSLPGYESFGTIVITYSMKAGIQTEEHPNPGKRYPGIQRTAYLPDNKEGRKVLKLLYRAFDQKLIFTVGYSRVLGVSDVITWNDIHHKTSRFGGPEMYGYPDPSYLKRVKEELKAKGIE

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 deltex 3-like (Drosophila)
别名 BBAP, B-lymphoma- and BAL-associated protein, deltex 3 like (Drosophila), E3 ubiquitin-protein ligase DTX3L, EC:2.3.2.27
计算分子量 740 aa, 84 kDa
观测分子量 84 kDa
GenBank蛋白编号BC060509
基因名称 DTX3L
Gene ID (NCBI) 151636
RRIDAB_874119
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ8TDB6
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
IHC protocol for DTX3L antibody 11963-1-APDownload protocol
WB protocol for DTX3L antibody 11963-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanIHC

Int J Biol Macromol

E3 ubiquitin ligase DTX3L promotes breast cancer progression by enhancing PKCα ubiquitination and inhibiting the p38 MAPK signaling pathway.

Authors - Xiaoxiao Zhang
View Article
humanWB

Cancer Cell Int

Circular RNA circEYA3 promotes the radiation resistance of hepatocellular carcinoma via the IGF2BP2/DTX3L axis

Authors - Pan Hu
View Article
goatIHC

Biomolecules

Differential MicroRNA Expression Involved in Endometrial Receptivity of Goats.

Authors - Xupeng Zang
View Article
humanWB

Cancer Sci

Ubiquitination of NOTCH2 by DTX3 suppresses the proliferation and migration of human esophageal carcinoma.

Authors - Xin-Yu Ding
View Article
humanWB,IHC

Sci Rep

Pan-cancer analysis of DTX3L as a potential prognostic and immunological biomarker.

Authors - Yunfeng Zhang
  • KD Validated
View Article
humanWB

Sci Rep

DTX3L promotes ovarian cancer progression and cisplatin resistance by activating JAK1/STAT1/OAS3 pathway.

Authors - Fangfang Lu
  • KD Validated
View Article
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