验证数据展示
经过测试的应用
| Positive WB detected in | mouse kidney tissue, mouse ovary tissue, mouse testis tissue, mouse brain tissue, hTERT-RPE1 cells, rat testis tissue |
| Positive IHC detected in | human kidney tissue, human colon tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| KD/KO | See 2 publications below |
| WB | See 2 publications below |
产品信息
25304-1-AP targets DTX3 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.
| 经测试应用 | WB, IHC, ELISA Application Description |
| 文献引用应用 | WB |
| 经测试反应性 | human, mouse |
| 文献引用反应性 | human |
| 免疫原 |
CatNo: Ag18111 Product name: Recombinant human DTX3 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-249 aa of BC114498 Sequence: MSFVLSRMAACGGTCKNKVTVSKPVWDFLSKETPARLARLREEHRVSILIDGETSDIYVLQLSPQGPPPAPPNGLYLARKALKGLLKEAEKELKKAQRQGELMGCLALGGGGEHPEMHRAGPPPLRAAPLLPPGARGLPPPPPPLPPPLPPRLREEAEEQESTCPICLGEIQNAKTLEKCRHSFCEGCITRALQVKKACPMCGRFYGQLVGNQPQNGRMLVSKDATLLLPSYEKYGTIVIQYVFPPGVQ 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | deltex homolog 3 (Drosophila) |
| 别名 | deltex homolog 3 (Drosophila), Deltex3, DTX3, Protein deltex 3, RING finger protein 154, RNF154 |
| 计算分子量 | 347 aa, 38 kDa |
| 观测分子量 | 38 kDa |
| GenBank蛋白编号 | BC114498 |
| 基因名称 | DTX3 |
| Gene ID (NCBI) | 196403 |
| RRID | AB_2880021 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q8N9I9 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
DTX3, also named as Deltex3 or RING finger protein 154, is 347 amino acid protein, which contains one RING-type zinc finger and belongs to the Deltex family. DTX3 is a regulator of Notch signaling, which is involved in cell-cell communications that regulates a broad spectrum of cell-fate determinations.
实验方案
| Product Specific Protocols | |
|---|---|
| IHC protocol for DTX3 antibody 25304-1-AP | Download protocol |
| WB protocol for DTX3 antibody 25304-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
J Cancer Deltex3 inhibits Epithelial Mesenchymal Transition in Papillary Thyroid Carcinoma via promoting ubiquitination of XRCC5 to regulate the AKT signal pathway.
| ||
Mol Biol Rep Deltex E3 ubiquitin ligase 3 inhibits colorectal cancer cell growth and regulates cell cycle progression via upregulating E2F transcription factor 1.
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