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  • Featured Product
  • KD/KO Validated

DTX3 Polyclonal antibody

DTX3 Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IHC, WB, ELISA

Conjugate

Unconjugated

Cat No : 25304-1-AP

Print datasheet

Synonyms

deltex homolog 3 (Drosophila), Deltex3, DTX3, Protein deltex 3, RING finger protein 154, RNF154

验证数据展示

  • WB analysis using 25304-1-AP

    WB analysis using 25304-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 25304-1-AP (DTX3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • WB analysis of hTERT-RPE1 using 25304-1-AP

    WB analysis of hTERT-RPE1 using 25304-1-AP

    Western blot analysis in wildtype and DTX3 knockouted human hTERT-RPE1 cells with 25304-1-AP (DTX3 antibody). The data provided by an anonymous customer.

  • WB analysis using 25304-1-AP

    WB analysis using 25304-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 25304-1-AP (DTX3 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • IHC staining of human colon using 25304-1-AP

    IHC staining of human colon using 25304-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 25304-1-AP (DTX3 Antibody) at dilution of 1:50 (under 40x lens).

  • IHC staining of human kidney using 25304-1-AP

    IHC staining of human kidney using 25304-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 25304-1-AP (DTX3 Antibody) at dilution of 1:50 (under 40x lens).

经过测试的应用

Positive WB detected inmouse kidney tissue, mouse ovary tissue, mouse testis tissue, mouse brain tissue, hTERT-RPE1 cells, rat testis tissue
Positive IHC detected inhuman kidney tissue, human colon tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

25304-1-AP targets DTX3 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications IHC, WB, ELISA
Cited ApplicationsWB
Tested Reactivity human, mouse
Cited Reactivityhuman
Immunogen DTX3 fusion protein Ag18111 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name deltex homolog 3 (Drosophila)
Synonyms deltex homolog 3 (Drosophila), Deltex3, DTX3, Protein deltex 3, RING finger protein 154, RNF154
Calculated Molecular Weight 347 aa, 38 kDa
Observed Molecular Weight 38 kDa
GenBank Accession NumberBC114498
Gene Symbol DTX3
Gene ID (NCBI) 196403
RRIDAB_2880021
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8N9I9
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

DTX3, also named as Deltex3 or RING finger protein 154, is 347 amino acid protein, which contains one RING-type zinc finger and belongs to the Deltex family. DTX3 is a regulator of Notch signaling, which is involved in cell-cell communications that regulates a broad spectrum of cell-fate determinations.

实验方案

Product Specific Protocols
WB protocol for DTX3 antibody 25304-1-APDownload protocol
IHC protocol for DTX3 antibody 25304-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

J Cancer

Deltex3 inhibits Epithelial Mesenchymal Transition in Papillary Thyroid Carcinoma via promoting ubiquitination of XRCC5 to regulate the AKT signal pathway.

Authors - Lidong Wang
  • KD Validated
View Article
humanWB

Mol Biol Rep

Deltex E3 ubiquitin ligase 3 inhibits colorectal cancer cell growth and regulates cell cycle progression via upregulating E2F transcription factor 1.

Authors - Hongli Xu
  • KO Validated
View Article