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验证数据展示

  • IF Staining of HeLa using 83107-1-RR

    IF Staining of HeLa using 83107-1-RR

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using DTL antibody (83107-1-RR, Clone: 230144C9 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1), CL594-Phalloidin (red).

  • FC experiment of HeLa using 83107-1-RR

    FC experiment of HeLa using 83107-1-RR

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug DTL Recombinant antibody (83107-1-RR, Clone:230144C9) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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DTL Recombinant monoclonal antibody

DTL Uni-rAb® Recombinant Antibody for IF/ICC, FC (Intra), ELISA
Cat No. 83107-1-RR

产品说明书

CloneNo. 230144C9

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

IF/ICC, FC (Intra), ELISA

别名

Denticleless protein homolog, DDB1- and CUL4-associated factor 2, DCAF2, CDW1, CDT2

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive IF/ICC detected inHeLa cells
Positive FC (Intra) detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy® DTL Ready-To-Use IHC Kit. DTL primary antibody included.

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

83107-1-RR targets DTL in IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.

经测试应用 IF/ICC, FC (Intra), ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag33391

Product name: Recombinant human DTL protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-240 aa of BC033540

Sequence: MLFNSVLRQPQLGVLRNGWSSQYPLQSLLTGYQCSGNDEHTSYGETGVPVPPFGCTFSSAPNMEHVLAVANEEGFVRLYNTESQSFRKKCFKEWMAHWNAVFDLAWVPGELKLVTAAGDQTAKFWDVKAGELIGTCKGHQCSLKSVAFSKFEKAVFCTGGRDGNIMVWDTRCNKKDGFYRQVNQISGAHNTSDKQTPSKPKKKQNSKGLAPSVDFQQSVTVVLFQDENTLVSAGAVDGII

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 denticleless homolog (Drosophila)
别名 Denticleless protein homolog, DDB1- and CUL4-associated factor 2, DCAF2, CDW1, CDT2
计算分子量 730 aa, 79 kDa
GenBank蛋白编号BC033540
基因名称 DTL
Gene ID (NCBI) 51514
RRIDAB_3670819
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDQ9NZJ0
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CDT2, also named as L2DTL or RAMP, is one of the substrate receptors of the Cullin Ring Ubiquitin Ligase 4 that targets for ubiquitin mediated degradation a number of substrates, such as CDT1, p21 and CHK1, involved in the regulation of cell cycle and survival. CDT2 overexpression was reported in breast (PMID: 18542055), gastric (PMID: 19672268) and ovarian carcinomas (PMID: 23995842) and rhabdomyosarcomas (PMID: 19235922) and associated with the aggressiveness of hepatocellular carcinomas (PMID: 17106265).

实验方案

Product Specific Protocols
IF protocol for DTL antibody 83107-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols